Galactofuranans and Galactomannan of Cell Walls as Chemotaxonomic Characteristics of the Genus and Species of Clavibacter

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Abstract

Two types of glycopolymers of different structures were found in the cell walls of type strains of two species of phytopathogenic bacteria, Clavibacter insidiosus VKM Ac-1402T and Clavibacter nebraskensis VKM Ac-1404T (family Microbacteriaceae, class Actinomycetes). The first type is represented by new, previously undescribed (1→6)-linked β-D-galactofuranans, which differ in the studied strains of the two species by the structure of side oligosaccharide chains (the structures are given in the text). The structure of the second glycopolymer, pyruvate-containing galactomannan, was identical in both strains. The results obtained in this work, together with those previously obtained, indicate that the presence of pyruvate-containing galactomannan and galactofuranans with an identical core structure and different oligosaccharide side substituents can be considered as a chemotaxonomic trait of the genus Clavibacter, and galactofuranans with di-, tri-, or tetrasaccharide substituents of different composition and structures can serve as chemotaxonomic markers of species. The data obtained expand our understanding of the structural diversity of natural glycopolymers and structural features of the bacteria cell walls of various taxa and may be of interest for taxonomic studies and studies aimed at elucidating the molecular mechanisms of interaction between bacteria and plant cells.

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About the authors

E. M. Tulskaya

Moscow State University

Email: em_tulskaya@mail.ru
Russian Federation, Moscow

D. Kim

Moscow State University

Email: em_tulskaya@mail.ru
Russian Federation, Moscow

N. V. Potekhina

Moscow State University

Email: em_tulskaya@mail.ru
Russian Federation, Moscow

A. S. Shashkov

Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences

Author for correspondence.
Email: em_tulskaya@mail.ru
Russian Federation, Moscow

S. N. Senchenkova

Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences

Email: em_tulskaya@mail.ru
Russian Federation, Moscow

L. V. Dorofeeva

All-Russian Collection of Microorganisms (VKM), Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences

Email: em_tulskaya@mail.ru
Russian Federation, Pushchino

L. I. Evtushenko

3All-Russian Collection of Microorganisms (VKM), Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research, Russian Academy of Sciences

Email: em_tulskaya@mail.ru
Russian Federation, Pushchino

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2. Figure. 13C NMR spectrum of the "cold extraction" preparation from the cell wall of C. insidiosus VKM As-1402T. The Arabic numerals in the numbers refer to the carbon atoms in the residues labeled with capital letters in Table 1

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