Extracellular Zn Detoxication by  Penicillium chrysogenum  and  Aspergillus niger

K. V. Sazanova; Сазанова К. В.; M. S. Zelenskaya; Зеленская М. С.; A. V. Korneev; Корнеев А. В.; D. Yu. Vlasov; Власов Д. Ю.

doi:10.31857/S0026364823060089

Extracellular Zn Detoxication by Penicillium chrysogenum and Aspergillus niger

Authors: Sazanova K.V.¹^,2^,3, Zelenskaya M.S.², Korneev A.V.², Vlasov D.Y.¹^,2
Affiliations:
1. Komarov Botanical Institute of the Russian Academy of Science
2. St. Petersburg State University, 199034
3. Archive of the Russian Academy of Sciences, St. Petersburg Branch
Issue: Vol 57, No 6 (2023)
Pages: 425-434
Section: ФИЗИОЛОГИЯ, БИОХИМИЯ, БИОТЕХНОЛОГИЯ
URL: https://journals.rcsi.science/0026-3648/article/view/231898
DOI: https://doi.org/10.31857/S0026364823060089
EDN: https://elibrary.ru/TXQBNH
ID: 231898

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Abstract
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Abstract

Microfungi are promising objects for bioremediation due to their ability to convert metals into forms that are less mobile and accessible to organisms. The study showed that the fungi Penicillium chrysogenum and Aspergillus niger have physiological mechanisms of extracellular Zn detoxification at its initial concentration of 250 µmol – 2 mmol in the medium. At concentrations of 250–500 µmol Zn promotes the accumulation of biomass and abundant sporulation of A. niger and Penicillium chrysogenum, and at concentrations of 1–2 mmol inhibits the growth of fungi. Extracellular Zn detoxification in Aspergillus niger culture occurs by the formation of dihydrate zinc oxalate katsarosite, due to the active biosynthesis of oxalic acid in A. niger. The main mechanism of zinc detoxification in Penicillium chrysogenum culture was the formation of zinc phosphate hopeite. The formation of zinc phosphate hopeite under the action of the fungus was established for the first time. The key factors that determine the direction of the processes of formation of extracellular mineral phases are the amounts of EPS and oxalic acid produced by fungi, as well as changes in the pH of the medium during the growth of cultures.

Keywords

bioremediation, detoxification, extracellular polymer matrix, heavy metals, organic acids, oxalates

References

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