Metagenomic microbial community profiling of Unnai hot spring by Ion-Torrent based shotgun sequencing
- Authors: Mangrola A.V.1, Dudhagara P.R.2, Koringa P.G.3, Joshi C.G.3, Patel R.K.4
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Affiliations:
- Department of Biochemistry
- Department of Biosciences
- Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry
- Department of Life Sciences
- Issue: Vol 87, No 1 (2018)
- Pages: 143-146
- Section: Experimental Articles
- URL: https://journals.rcsi.science/0026-2617/article/view/163393
- DOI: https://doi.org/10.1134/S0026261718010113
- ID: 163393
Cite item
Abstract
This is the first report on depicting the pioneering microbiota of Unnai hot spring using shotgun metagenome sequencing approach. Community analysis encompassed a total of 688,059 sequences with the total size 125.31 Mbp and 46% G + C content. Sequencing metagenome reported about 992 species belonged to 40 different phyla dominated by Firmicutes (97.49%), Proteobacteria (1.36%), and Actinobacteria (0.31%). In functional analysis, Non-Supervised Orthologous Groups (NOG) annotation revealed the predominance of poorly characterized reads (82.79%). Moreover, the subsystem classification displayed 19% genes assigned to carbohydrates metabolism, 12% genes allocated to clustering-based subsystems, 10% genes belonged to amino acids and its derivatives. The result suggests the huge bacterial diversity which will be useful for further characterizing the economically important bacteria for biotechnological applications.
Keywords
About the authors
A. V. Mangrola
Department of Biochemistry
Author for correspondence.
Email: avmang@gmail.com
India, Anand, Gujarat
P. R. Dudhagara
Department of Biosciences
Email: avmang@gmail.com
India, Surat, Gujarat
P. G. Koringa
Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry
Email: avmang@gmail.com
India, Anand, Gujarat
C. G. Joshi
Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry
Email: avmang@gmail.com
India, Anand, Gujarat
R. K. Patel
Department of Life Sciences
Email: avmang@gmail.com
India, Patan, Gujarat
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