Comparative analysis of esterase and paraoxonase activities of different serum albumin species
- Authors: Goncharov N.V.1,2, Terpilovskii M.A.1, Shmurak V.I.2, Belinskaya D.A.1, Avdonin P.V.3
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Affiliations:
- Sechenov Institute of Evolutionary Physiology and Biochemistry
- Research Institute of Hygiene
- Koltsov Institute of Developmental Biology
- Issue: Vol 53, No 4 (2017)
- Pages: 271-281
- Section: Comparative and Ontogenic Biochemistry
- URL: https://journals.rcsi.science/0022-0930/article/view/159280
- DOI: https://doi.org/10.1134/S0022093017040032
- ID: 159280
Cite item
Abstract
Enzymatic activities of three types of serum albumin—rat, bovine and human—were analyzed comparatively using a mathematical model. Kinetic and equilibrium constants of carboxylesterase and paraoxonase activities of albumin in Sudlow’s sites I and II were determined. The effects of specific ligands, ibuprofen and warfarin, on enzyme kinetics in these sites were studied. Ibuprofen was found to have an inhibitory effect both on carboxylesterase and paraoxonase albumin activities, whereas warfarin specifically inhibited only carboxylesterase albumin activity.
About the authors
N. V. Goncharov
Sechenov Institute of Evolutionary Physiology and Biochemistry; Research Institute of Hygiene
Author for correspondence.
Email: ngoncharov@gmail.com
Russian Federation, St. Petersburg; St. Petersburg
M. A. Terpilovskii
Sechenov Institute of Evolutionary Physiology and Biochemistry
Email: ngoncharov@gmail.com
Russian Federation, St. Petersburg
V. I. Shmurak
Research Institute of Hygiene
Email: ngoncharov@gmail.com
Russian Federation, St. Petersburg
D. A. Belinskaya
Sechenov Institute of Evolutionary Physiology and Biochemistry
Email: ngoncharov@gmail.com
Russian Federation, St. Petersburg
P. V. Avdonin
Koltsov Institute of Developmental Biology
Email: ngoncharov@gmail.com
Russian Federation, Moscow