HPLC determination of tetracycline antibiotics in milk with post-column derivatization and fluorescence detection
- Авторы: Kargin I.D.1, Sokolova L.S.1, Pirogov A.V.1, Shpigun O.A.1
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Учреждения:
- Moscow State University
- Выпуск: Том 52, № 14 (2016)
- Страницы: 1365-1369
- Раздел: Analysis of Substances
- URL: https://journals.rcsi.science/0020-1685/article/view/158094
- DOI: https://doi.org/10.1134/S0020168516140065
- ID: 158094
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Аннотация
The conditions for sensitive and selective determination of tetracyclines in milk in the form of their complexes with Mg2+ via the HPLC method with post-column derivatization and fluorescence detection are found. It is shown that the fluorescence of tetracycline-Mg2+ complexes in microemulsions is 1.8 times more intense than that in aqueous acetonitrile medium. The detection limits of tetracycline, oxytetracycline, and doxycycline are 5, 8 and 25 ng mL–1, respectively.
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Об авторах
I. Kargin
Moscow State University
Автор, ответственный за переписку.
Email: karginid@gmail.com
Россия, Moscow
L. Sokolova
Moscow State University
Email: karginid@gmail.com
Россия, Moscow
A. Pirogov
Moscow State University
Email: karginid@gmail.com
Россия, Moscow
O. Shpigun
Moscow State University
Email: karginid@gmail.com
Россия, Moscow
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