HPLC determination of tetracycline antibiotics in milk with post-column derivatization and fluorescence detection

I. D. Kargin; L. S. Sokolova; A. V. Pirogov; O. A. Shpigun

doi:10.1134/S0020168516140065

HPLC determination of tetracycline antibiotics in milk with post-column derivatization and fluorescence detection

Authors: Kargin I.D.¹, Sokolova L.S.¹, Pirogov A.V.¹, Shpigun O.A.¹
Affiliations:
1. Moscow State University
Issue: Vol 52, No 14 (2016)
Pages: 1365-1369
Section: Analysis of Substances
URL: https://journals.rcsi.science/0020-1685/article/view/158094
DOI: https://doi.org/10.1134/S0020168516140065
ID: 158094

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Abstract

The conditions for sensitive and selective determination of tetracyclines in milk in the form of their complexes with Mg²⁺ via the HPLC method with post-column derivatization and fluorescence detection are found. It is shown that the fluorescence of tetracycline-Mg²⁺ complexes in microemulsions is 1.8 times more intense than that in aqueous acetonitrile medium. The detection limits of tetracycline, oxytetracycline, and doxycycline are 5, 8 and 25 ng mL^–1, respectively.