Thermostable Recombinant Polypeptides as the Source of L-Amino Acids for Culture Media
- 作者: Grishin D.1, Zhdanov D.1, Gladilina Y.1, Podobed O.1, Pokrovsky V.1, Pokrovskaya M.1, Aleksandrova S.1, Sokolov N.1
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隶属关系:
- V. N. Orekhovich Research Institute of Biomedical Chemistry
- 期: 卷 165, 编号 4 (2018)
- 页面: 461-464
- 栏目: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/240419
- DOI: https://doi.org/10.1007/s10517-018-4194-7
- ID: 240419
如何引用文章
详细
Mutant homologues of small chemotactic and DNA-binding proteins from thermophilic bacteria Thermotoga petrophila RKU-1 and Thermotoga naphthophila were obtained. These proteins can be expressed in the recombinant form in E. coli cells. A wide range of properties and parameters that are important for isolation of these proteins were revealed: stability in a wide temperature and pH range, high level of expression, solubility, and the possibility of using simple purification schemes with low number of successive steps. The positive effect of proteins on in vitro fibroblasts growth was demonstrated. The described properties of the target proteins indicate the possibility of their use in different biotechnology industries as an inexpensive source of L-amino acids.
作者简介
D. Grishin
V. N. Orekhovich Research Institute of Biomedical Chemistry
编辑信件的主要联系方式.
Email: molbiol_ibm@inbox.ru
俄罗斯联邦, Moscow
D. Zhdanov
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
俄罗斯联邦, Moscow
Yu. Gladilina
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
俄罗斯联邦, Moscow
O. Podobed
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
俄罗斯联邦, Moscow
V. Pokrovsky
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
俄罗斯联邦, Moscow
M. Pokrovskaya
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
俄罗斯联邦, Moscow
S. Aleksandrova
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
俄罗斯联邦, Moscow
N. Sokolov
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
俄罗斯联邦, Moscow
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