Thermostable Recombinant Polypeptides as the Source of L-Amino Acids for Culture Media
- Авторлар: Grishin D.1, Zhdanov D.1, Gladilina Y.1, Podobed O.1, Pokrovsky V.1, Pokrovskaya M.1, Aleksandrova S.1, Sokolov N.1
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Мекемелер:
- V. N. Orekhovich Research Institute of Biomedical Chemistry
- Шығарылым: Том 165, № 4 (2018)
- Беттер: 461-464
- Бөлім: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/240419
- DOI: https://doi.org/10.1007/s10517-018-4194-7
- ID: 240419
Дәйексөз келтіру
Аннотация
Mutant homologues of small chemotactic and DNA-binding proteins from thermophilic bacteria Thermotoga petrophila RKU-1 and Thermotoga naphthophila were obtained. These proteins can be expressed in the recombinant form in E. coli cells. A wide range of properties and parameters that are important for isolation of these proteins were revealed: stability in a wide temperature and pH range, high level of expression, solubility, and the possibility of using simple purification schemes with low number of successive steps. The positive effect of proteins on in vitro fibroblasts growth was demonstrated. The described properties of the target proteins indicate the possibility of their use in different biotechnology industries as an inexpensive source of L-amino acids.
Авторлар туралы
D. Grishin
V. N. Orekhovich Research Institute of Biomedical Chemistry
Хат алмасуға жауапты Автор.
Email: molbiol_ibm@inbox.ru
Ресей, Moscow
D. Zhdanov
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
Ресей, Moscow
Yu. Gladilina
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
Ресей, Moscow
O. Podobed
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
Ресей, Moscow
V. Pokrovsky
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
Ресей, Moscow
M. Pokrovskaya
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
Ресей, Moscow
S. Aleksandrova
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
Ресей, Moscow
N. Sokolov
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
Ресей, Moscow
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