Thermostable Recombinant Polypeptides as the Source of L-Amino Acids for Culture Media
- Authors: Grishin D.V.1, Zhdanov D.D.1, Gladilina Y.A.1, Podobed O.V.1, Pokrovsky V.S.1, Pokrovskaya M.V.1, Aleksandrova S.S.1, Sokolov N.N.1
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Affiliations:
- V. N. Orekhovich Research Institute of Biomedical Chemistry
- Issue: Vol 165, No 4 (2018)
- Pages: 461-464
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/240419
- DOI: https://doi.org/10.1007/s10517-018-4194-7
- ID: 240419
Cite item
Abstract
Mutant homologues of small chemotactic and DNA-binding proteins from thermophilic bacteria Thermotoga petrophila RKU-1 and Thermotoga naphthophila were obtained. These proteins can be expressed in the recombinant form in E. coli cells. A wide range of properties and parameters that are important for isolation of these proteins were revealed: stability in a wide temperature and pH range, high level of expression, solubility, and the possibility of using simple purification schemes with low number of successive steps. The positive effect of proteins on in vitro fibroblasts growth was demonstrated. The described properties of the target proteins indicate the possibility of their use in different biotechnology industries as an inexpensive source of L-amino acids.
About the authors
D. V. Grishin
V. N. Orekhovich Research Institute of Biomedical Chemistry
Author for correspondence.
Email: molbiol_ibm@inbox.ru
Russian Federation, Moscow
D. D. Zhdanov
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
Russian Federation, Moscow
Yu. A. Gladilina
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
Russian Federation, Moscow
O. V. Podobed
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
Russian Federation, Moscow
V. S. Pokrovsky
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
Russian Federation, Moscow
M. V. Pokrovskaya
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
Russian Federation, Moscow
S. S. Aleksandrova
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
Russian Federation, Moscow
N. N. Sokolov
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
Russian Federation, Moscow