Isolation and Characterization of Human Myoblast Culture In Vitro for Technologies of Cell and Gene Therapy of Skeletal Muscle Pathologies
- 作者: Tabakov V.1, Zinov’eva O.2, Voskresenskaya O.2, Skoblov M.1
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隶属关系:
- Research Centre for Medical Genetics
- Department of Nervous Diseases, I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation
- 期: 卷 164, 编号 4 (2018)
- 页面: 536-542
- 栏目: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/239734
- DOI: https://doi.org/10.1007/s10517-018-4028-7
- ID: 239734
如何引用文章
详细
We analyzed cultures of 5 independent myoblast lines from human skeletal muscles. It was shown that the content of desmin-positive cells in cultures at early passages exceeds 90%. Typical morphofunctional signs of myogenic differentiation disturbances were identified and their dynamics was studied. Signs of alternative adipogenic and chondrogenic differentiation of cells were revealed. Based on these data, limitations for the use of myoblast cultures of certain passages for biomedical research and cell therapy were evaluated.
作者简介
V. Tabakov
Research Centre for Medical Genetics
编辑信件的主要联系方式.
Email: vyutab@yandex.ru
俄罗斯联邦, Moscow
O. Zinov’eva
Department of Nervous Diseases, I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation
Email: vyutab@yandex.ru
俄罗斯联邦, Moscow
O. Voskresenskaya
Department of Nervous Diseases, I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation
Email: vyutab@yandex.ru
俄罗斯联邦, Moscow
M. Skoblov
Research Centre for Medical Genetics
Email: vyutab@yandex.ru
俄罗斯联邦, Moscow
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