Email this article Isolation and Characterization of Human Myoblast Culture In Vitro for Technologies of Cell and Gene Therapy of Skeletal Muscle Pathologies
- Authors: Tabakov V.Y.1, Zinov’eva O.E.2, Voskresenskaya O.N.2, Skoblov M.Y.1
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Affiliations:
- Research Centre for Medical Genetics
- Department of Nervous Diseases, I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation
- Issue: Vol 164, No 4 (2018)
- Pages: 536-542
- Section: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/239734
- DOI: https://doi.org/10.1007/s10517-018-4028-7
- ID: 239734
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Abstract
We analyzed cultures of 5 independent myoblast lines from human skeletal muscles. It was shown that the content of desmin-positive cells in cultures at early passages exceeds 90%. Typical morphofunctional signs of myogenic differentiation disturbances were identified and their dynamics was studied. Signs of alternative adipogenic and chondrogenic differentiation of cells were revealed. Based on these data, limitations for the use of myoblast cultures of certain passages for biomedical research and cell therapy were evaluated.
About the authors
V. Yu. Tabakov
Research Centre for Medical Genetics
Author for correspondence.
Email: vyutab@yandex.ru
Russian Federation, Moscow
O. E. Zinov’eva
Department of Nervous Diseases, I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation
Email: vyutab@yandex.ru
Russian Federation, Moscow
O. N. Voskresenskaya
Department of Nervous Diseases, I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation
Email: vyutab@yandex.ru
Russian Federation, Moscow
M. Yu. Skoblov
Research Centre for Medical Genetics
Email: vyutab@yandex.ru
Russian Federation, Moscow
