Isolation and Characterization of Human Myoblast Culture In Vitro for Technologies of Cell and Gene Therapy of Skeletal Muscle Pathologies
- Авторлар: Tabakov V.1, Zinov’eva O.2, Voskresenskaya O.2, Skoblov M.1
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Мекемелер:
- Research Centre for Medical Genetics
- Department of Nervous Diseases, I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation
- Шығарылым: Том 164, № 4 (2018)
- Беттер: 536-542
- Бөлім: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/239734
- DOI: https://doi.org/10.1007/s10517-018-4028-7
- ID: 239734
Дәйексөз келтіру
Аннотация
We analyzed cultures of 5 independent myoblast lines from human skeletal muscles. It was shown that the content of desmin-positive cells in cultures at early passages exceeds 90%. Typical morphofunctional signs of myogenic differentiation disturbances were identified and their dynamics was studied. Signs of alternative adipogenic and chondrogenic differentiation of cells were revealed. Based on these data, limitations for the use of myoblast cultures of certain passages for biomedical research and cell therapy were evaluated.
Авторлар туралы
V. Tabakov
Research Centre for Medical Genetics
Хат алмасуға жауапты Автор.
Email: vyutab@yandex.ru
Ресей, Moscow
O. Zinov’eva
Department of Nervous Diseases, I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation
Email: vyutab@yandex.ru
Ресей, Moscow
O. Voskresenskaya
Department of Nervous Diseases, I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation
Email: vyutab@yandex.ru
Ресей, Moscow
M. Skoblov
Research Centre for Medical Genetics
Email: vyutab@yandex.ru
Ресей, Moscow
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