Characteristics of Trophoblasts in Long-Term Culture
- 作者: Kolokol’tsova T.1,2, Saburina I.1,3, Nanovskaya T.2, Patrikeeva S.2, Vernikovskaya D.2, Zurina I.1, Gorkun A.1, Kosheleva N.1, Poltavtseva R.4, Sukhikh G.4
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隶属关系:
- Research Institute of General Pathology and Pathophysiology
- University of Texas Medical Branch
- Russian Medical Academy of Postgraduate Education, Ministry of Health of the Russian Federation
- V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- 期: 卷 164, 编号 2 (2017)
- 页面: 259-265
- 栏目: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/239541
- DOI: https://doi.org/10.1007/s10517-017-3969-6
- ID: 239541
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详细
We analyzed more than 40 cytotrophoblast cultures derived from cell islets that grew from trypsinized tissue fragments of placental microvilli. Phenotypic variability of trophoblasts was demonstrated. Changes in trophoblast morphology from epithelium-like or oval cells to bipolar and spindle-shaped or twisted and then to mesenchymal-like cells as well as intensive expression of cytokeratin-7 and vimentin attested to epithelial-mesenchymal transition of trophoblasts during in vitro culturing. Analysis of the expression of specific markers in long-term trophoblast culture (≥7 passages) revealed the possibility of culture contamination with other non-trophoblast cells including fibroblasts. High risk of trophoblast culture contamination with rapidly growing cells necessitates regular control of the cultures used in fundamental studies. Our experiments confirmed the possibility of long-term culturing of cells maintaining trophoblast properties. The identity and purity of 4 trophoblast cultures free from contamination and retaining the properties of pure culture during long-term (>10 passages) culturing in vitro were confirmed.
作者简介
T. Kolokol’tsova
Research Institute of General Pathology and Pathophysiology; University of Texas Medical Branch
编辑信件的主要联系方式.
Email: kolokoltd@mail.ru
俄罗斯联邦, Moscow; Galveston, Texas
I. Saburina
Research Institute of General Pathology and Pathophysiology; Russian Medical Academy of Postgraduate Education, Ministry of Health of the Russian Federation
Email: kolokoltd@mail.ru
俄罗斯联邦, Moscow; Moscow
T. Nanovskaya
University of Texas Medical Branch
Email: kolokoltd@mail.ru
美国, Galveston, Texas
S. Patrikeeva
University of Texas Medical Branch
Email: kolokoltd@mail.ru
美国, Galveston, Texas
D. Vernikovskaya
University of Texas Medical Branch
Email: kolokoltd@mail.ru
美国, Galveston, Texas
I. Zurina
Research Institute of General Pathology and Pathophysiology
Email: kolokoltd@mail.ru
俄罗斯联邦, Moscow
A. Gorkun
Research Institute of General Pathology and Pathophysiology
Email: kolokoltd@mail.ru
俄罗斯联邦, Moscow
N. Kosheleva
Research Institute of General Pathology and Pathophysiology
Email: kolokoltd@mail.ru
俄罗斯联邦, Moscow
R. Poltavtseva
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: kolokoltd@mail.ru
俄罗斯联邦, Moscow
G. Sukhikh
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: kolokoltd@mail.ru
俄罗斯联邦, Moscow