Characteristics of Trophoblasts in Long-Term Culture
- Авторлар: Kolokol’tsova T.1,2, Saburina I.1,3, Nanovskaya T.2, Patrikeeva S.2, Vernikovskaya D.2, Zurina I.1, Gorkun A.1, Kosheleva N.1, Poltavtseva R.4, Sukhikh G.4
-
Мекемелер:
- Research Institute of General Pathology and Pathophysiology
- University of Texas Medical Branch
- Russian Medical Academy of Postgraduate Education, Ministry of Health of the Russian Federation
- V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- Шығарылым: Том 164, № 2 (2017)
- Беттер: 259-265
- Бөлім: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/239541
- DOI: https://doi.org/10.1007/s10517-017-3969-6
- ID: 239541
Дәйексөз келтіру
Аннотация
We analyzed more than 40 cytotrophoblast cultures derived from cell islets that grew from trypsinized tissue fragments of placental microvilli. Phenotypic variability of trophoblasts was demonstrated. Changes in trophoblast morphology from epithelium-like or oval cells to bipolar and spindle-shaped or twisted and then to mesenchymal-like cells as well as intensive expression of cytokeratin-7 and vimentin attested to epithelial-mesenchymal transition of trophoblasts during in vitro culturing. Analysis of the expression of specific markers in long-term trophoblast culture (≥7 passages) revealed the possibility of culture contamination with other non-trophoblast cells including fibroblasts. High risk of trophoblast culture contamination with rapidly growing cells necessitates regular control of the cultures used in fundamental studies. Our experiments confirmed the possibility of long-term culturing of cells maintaining trophoblast properties. The identity and purity of 4 trophoblast cultures free from contamination and retaining the properties of pure culture during long-term (>10 passages) culturing in vitro were confirmed.
Авторлар туралы
T. Kolokol’tsova
Research Institute of General Pathology and Pathophysiology; University of Texas Medical Branch
Хат алмасуға жауапты Автор.
Email: kolokoltd@mail.ru
Ресей, Moscow; Galveston, Texas
I. Saburina
Research Institute of General Pathology and Pathophysiology; Russian Medical Academy of Postgraduate Education, Ministry of Health of the Russian Federation
Email: kolokoltd@mail.ru
Ресей, Moscow; Moscow
T. Nanovskaya
University of Texas Medical Branch
Email: kolokoltd@mail.ru
АҚШ, Galveston, Texas
S. Patrikeeva
University of Texas Medical Branch
Email: kolokoltd@mail.ru
АҚШ, Galveston, Texas
D. Vernikovskaya
University of Texas Medical Branch
Email: kolokoltd@mail.ru
АҚШ, Galveston, Texas
I. Zurina
Research Institute of General Pathology and Pathophysiology
Email: kolokoltd@mail.ru
Ресей, Moscow
A. Gorkun
Research Institute of General Pathology and Pathophysiology
Email: kolokoltd@mail.ru
Ресей, Moscow
N. Kosheleva
Research Institute of General Pathology and Pathophysiology
Email: kolokoltd@mail.ru
Ресей, Moscow
R. Poltavtseva
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: kolokoltd@mail.ru
Ресей, Moscow
G. Sukhikh
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: kolokoltd@mail.ru
Ресей, Moscow