Visualization of Mesenchymal Stromal Cells in 2Dand 3D-Cultures by Scanning Electron Microscopy with Lanthanide Contrasting
- 作者: Novikov I.1, Vakhrushev I.2, Antonov E.3, Yarygin K.2, Subbot A.1
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隶属关系:
- Institute of Eye Diseases
- V. N. Orekhovich Research Institute of Biomedical Chemistry
- Institute on Laser and Information Technologies, Russian Academy of Sciences
- 期: 卷 162, 编号 4 (2017)
- 页面: 558-562
- 栏目: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/238373
- DOI: https://doi.org/10.1007/s10517-017-3659-4
- ID: 238373
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详细
Mesenchymal stromal cells from deciduous teeth in 2D- and 3D-cultures on culture plastic, silicate glass, porous polystyrene, and experimental polylactoglycolide matrices were visualized by scanning electron microscopy with lanthanide contrasting. Supravital staining of cell cultures with a lanthanide-based dye (neodymium chloride) preserved normal cell morphology and allowed assessment of the matrix properties of the carriers. The developed approach can be used for the development of biomaterials for tissue engineering.
作者简介
I. Novikov
Institute of Eye Diseases
Email: kletkagb@gmail.com
俄罗斯联邦, Moscow
I. Vakhrushev
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: kletkagb@gmail.com
俄罗斯联邦, Moscow
E. Antonov
Institute on Laser and Information Technologies, Russian Academy of Sciences
Email: kletkagb@gmail.com
俄罗斯联邦, Moscow
K. Yarygin
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: kletkagb@gmail.com
俄罗斯联邦, Moscow
A. Subbot
Institute of Eye Diseases
编辑信件的主要联系方式.
Email: kletkagb@gmail.com
俄罗斯联邦, Moscow