Enviar artigo por via de e-mail Visualization of Mesenchymal Stromal Cells in 2Dand 3D-Cultures by Scanning Electron Microscopy with Lanthanide Contrasting
- Autores: Novikov I.A.1, Vakhrushev I.V.2, Antonov E.N.3, Yarygin K.N.2, Subbot A.M.1
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Afiliações:
- Institute of Eye Diseases
- V. N. Orekhovich Research Institute of Biomedical Chemistry
- Institute on Laser and Information Technologies, Russian Academy of Sciences
- Edição: Volume 162, Nº 4 (2017)
- Páginas: 558-562
- Seção: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/238373
- DOI: https://doi.org/10.1007/s10517-017-3659-4
- ID: 238373
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Resumo
Mesenchymal stromal cells from deciduous teeth in 2D- and 3D-cultures on culture plastic, silicate glass, porous polystyrene, and experimental polylactoglycolide matrices were visualized by scanning electron microscopy with lanthanide contrasting. Supravital staining of cell cultures with a lanthanide-based dye (neodymium chloride) preserved normal cell morphology and allowed assessment of the matrix properties of the carriers. The developed approach can be used for the development of biomaterials for tissue engineering.
Sobre autores
I. Novikov
Institute of Eye Diseases
Email: kletkagb@gmail.com
Rússia, Moscow
I. Vakhrushev
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: kletkagb@gmail.com
Rússia, Moscow
E. Antonov
Institute on Laser and Information Technologies, Russian Academy of Sciences
Email: kletkagb@gmail.com
Rússia, Moscow
K. Yarygin
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: kletkagb@gmail.com
Rússia, Moscow
A. Subbot
Institute of Eye Diseases
Autor responsável pela correspondência
Email: kletkagb@gmail.com
Rússia, Moscow
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