Email this article Visualization of Mesenchymal Stromal Cells in 2Dand 3D-Cultures by Scanning Electron Microscopy with Lanthanide Contrasting
- Authors: Novikov I.A.1, Vakhrushev I.V.2, Antonov E.N.3, Yarygin K.N.2, Subbot A.M.1
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Affiliations:
- Institute of Eye Diseases
- V. N. Orekhovich Research Institute of Biomedical Chemistry
- Institute on Laser and Information Technologies, Russian Academy of Sciences
- Issue: Vol 162, No 4 (2017)
- Pages: 558-562
- Section: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/238373
- DOI: https://doi.org/10.1007/s10517-017-3659-4
- ID: 238373
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Abstract
Mesenchymal stromal cells from deciduous teeth in 2D- and 3D-cultures on culture plastic, silicate glass, porous polystyrene, and experimental polylactoglycolide matrices were visualized by scanning electron microscopy with lanthanide contrasting. Supravital staining of cell cultures with a lanthanide-based dye (neodymium chloride) preserved normal cell morphology and allowed assessment of the matrix properties of the carriers. The developed approach can be used for the development of biomaterials for tissue engineering.
About the authors
I. A. Novikov
Institute of Eye Diseases
Email: kletkagb@gmail.com
Russian Federation, Moscow
I. V. Vakhrushev
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: kletkagb@gmail.com
Russian Federation, Moscow
E. N. Antonov
Institute on Laser and Information Technologies, Russian Academy of Sciences
Email: kletkagb@gmail.com
Russian Federation, Moscow
K. N. Yarygin
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: kletkagb@gmail.com
Russian Federation, Moscow
A. M. Subbot
Institute of Eye Diseases
Author for correspondence.
Email: kletkagb@gmail.com
Russian Federation, Moscow
