Production of Recombinant Human Transferrin in Eukaryotic Pichia pastoris Expression System
- Авторлар: Bobik T.1, Popov R.1, Aliev T.1, Mokrushina Y.1, Shamborant O.1, Khurs E.2, Knorre V.1, Smirnov I.1, Gabibov A.1
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Мекемелер:
- M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
- Шығарылым: Том 167, № 3 (2019)
- Беттер: 335-338
- Бөлім: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/241682
- DOI: https://doi.org/10.1007/s10517-019-04521-0
- ID: 241682
Дәйексөз келтіру
Аннотация
The development and manufacturing of serum-free culture media allowing reducing the costs of preparations and standardizing the biotechnological process are important trends in biotechnology. Substitution of protein compounds in the serum-free media with recombinant analogues reduces the risk of contamination with various infectious agents. Human transferrin is a protein component of serum-free media responsible for the transport of Fe3+ ions into cells. We generated a producing strain P. pastoris secreting human transferrin to the culture medium. The use of constitutive GAP promoter and maintenance of medium pH at 6.5 allows attaining maximum level of transferrin expression (20 mg/liter).
Авторлар туралы
T. Bobik
M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Хат алмасуға жауапты Автор.
Email: bobik_tanya@mail.ru
Ресей, Moscow
R. Popov
M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
Ресей, Moscow
T. Aliev
M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
Ресей, Moscow
Yu. Mokrushina
M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
Ресей, Moscow
O. Shamborant
M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
Ресей, Moscow
E. Khurs
V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
Ресей, Moscow
V. Knorre
M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
Ресей, Moscow
I. Smirnov
M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
Ресей, Moscow
A. Gabibov
M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
Ресей, Moscow