Production of Recombinant Human Transferrin in Eukaryotic Pichia pastoris Expression System
- 作者: Bobik T.1, Popov R.1, Aliev T.1, Mokrushina Y.1, Shamborant O.1, Khurs E.2, Knorre V.1, Smirnov I.1, Gabibov A.1
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隶属关系:
- M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
- 期: 卷 167, 编号 3 (2019)
- 页面: 335-338
- 栏目: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/241682
- DOI: https://doi.org/10.1007/s10517-019-04521-0
- ID: 241682
如何引用文章
详细
The development and manufacturing of serum-free culture media allowing reducing the costs of preparations and standardizing the biotechnological process are important trends in biotechnology. Substitution of protein compounds in the serum-free media with recombinant analogues reduces the risk of contamination with various infectious agents. Human transferrin is a protein component of serum-free media responsible for the transport of Fe3+ ions into cells. We generated a producing strain P. pastoris secreting human transferrin to the culture medium. The use of constitutive GAP promoter and maintenance of medium pH at 6.5 allows attaining maximum level of transferrin expression (20 mg/liter).
作者简介
T. Bobik
M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
编辑信件的主要联系方式.
Email: bobik_tanya@mail.ru
俄罗斯联邦, Moscow
R. Popov
M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
俄罗斯联邦, Moscow
T. Aliev
M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
俄罗斯联邦, Moscow
Yu. Mokrushina
M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
俄罗斯联邦, Moscow
O. Shamborant
M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
俄罗斯联邦, Moscow
E. Khurs
V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
俄罗斯联邦, Moscow
V. Knorre
M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
俄罗斯联邦, Moscow
I. Smirnov
M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
俄罗斯联邦, Moscow
A. Gabibov
M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
俄罗斯联邦, Moscow