Production of Recombinant Human Transferrin in Eukaryotic Pichia pastoris Expression System
- Authors: Bobik T.V.1, Popov R.Y.1, Aliev T.K.1, Mokrushina Y.A.1, Shamborant O.G.1, Khurs E.N.2, Knorre V.D.1, Smirnov I.V.1, Gabibov A.G.1
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Affiliations:
- M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
- Issue: Vol 167, No 3 (2019)
- Pages: 335-338
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/241682
- DOI: https://doi.org/10.1007/s10517-019-04521-0
- ID: 241682
Cite item
Abstract
The development and manufacturing of serum-free culture media allowing reducing the costs of preparations and standardizing the biotechnological process are important trends in biotechnology. Substitution of protein compounds in the serum-free media with recombinant analogues reduces the risk of contamination with various infectious agents. Human transferrin is a protein component of serum-free media responsible for the transport of Fe3+ ions into cells. We generated a producing strain P. pastoris secreting human transferrin to the culture medium. The use of constitutive GAP promoter and maintenance of medium pH at 6.5 allows attaining maximum level of transferrin expression (20 mg/liter).
About the authors
T. V. Bobik
M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Author for correspondence.
Email: bobik_tanya@mail.ru
Russian Federation, Moscow
R. Yu. Popov
M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
Russian Federation, Moscow
T. K. Aliev
M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
Russian Federation, Moscow
Yu. A. Mokrushina
M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
Russian Federation, Moscow
O. G. Shamborant
M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
Russian Federation, Moscow
E. N. Khurs
V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
Russian Federation, Moscow
V. D. Knorre
M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
Russian Federation, Moscow
I. V. Smirnov
M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
Russian Federation, Moscow
A. G. Gabibov
M. M. Schemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
Russian Federation, Moscow