Detection of Small Subsets of CD4 +  Lymphocytes with SmartFlare Nanoprobes

A. P. Toptygina; R. Sh. Zakirov; K. S. Kapitanova; E. L. Semikina

doi:10.1007/s10517-019-04689-5

Detection of Small Subsets of CD4⁺ Lymphocytes with SmartFlare Nanoprobes

Authors: Toptygina A.P.¹, Zakirov R.S.², Kapitanova K.S.¹, Semikina E.L.²
Affiliations:
1. G. N. Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology, Federal Service on Surveillance for Consumer Rights Protection and Human Well-Being
2. National Medical Research Center for Children’s Health, Ministry of Health of the Russian Federation
Issue: Vol 168, No 2 (2019)
Pages: 270-274
Section: Article
URL: https://journals.rcsi.science/0007-4888/article/view/242399
DOI: https://doi.org/10.1007/s10517-019-04689-5
ID: 242399

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Abstract
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Abstract

SmartFlare technology allows detection of mRNA in single living cells. We studied the possibility of using SmartFlare nanoprobes for detection of small subsets of CD4⁺ lymphocytes. It was found that SmartFlare allows detection of transcriptional master regulators of major CD4⁺T helper subsets in living human lymphocytes. Nanoprobes labeled with Cy5 fluorophore were better detected by flow cytometry than nanoprobes labeled with Cy3. Appropriate time of lymphocyte incubation with SmartFlare probes was 24 h.

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Detection of Small Subsets of CD4+ Lymphocytes with SmartFlare Nanoprobes

Full Text

Abstract

About the authors

A. P. Toptygina

R. Sh. Zakirov

K. S. Kapitanova

E. L. Semikina

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Detection of Small Subsets of CD4⁺ Lymphocytes with SmartFlare Nanoprobes