Отправить статью по E-mail Detection of Small Subsets of CD4+ Lymphocytes with SmartFlare Nanoprobes
- Авторы: Toptygina A.1, Zakirov R.2, Kapitanova K.1, Semikina E.2
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Учреждения:
- G. N. Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology, Federal Service on Surveillance for Consumer Rights Protection and Human Well-Being
- National Medical Research Center for Children’s Health, Ministry of Health of the Russian Federation
- Выпуск: Том 168, № 2 (2019)
- Страницы: 270-274
- Раздел: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/242399
- DOI: https://doi.org/10.1007/s10517-019-04689-5
- ID: 242399
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Аннотация
SmartFlare technology allows detection of mRNA in single living cells. We studied the possibility of using SmartFlare nanoprobes for detection of small subsets of CD4+ lymphocytes. It was found that SmartFlare allows detection of transcriptional master regulators of major CD4+T helper subsets in living human lymphocytes. Nanoprobes labeled with Cy5 fluorophore were better detected by flow cytometry than nanoprobes labeled with Cy3. Appropriate time of lymphocyte incubation with SmartFlare probes was 24 h.
Об авторах
A. Toptygina
G. N. Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology, Federal Service on Surveillance for Consumer Rights Protection and Human Well-Being
Автор, ответственный за переписку.
Email: toptyginaanna@rambler.ru
Россия, Moscow
R. Zakirov
National Medical Research Center for Children’s Health, Ministry of Health of the Russian Federation
Email: toptyginaanna@rambler.ru
Россия, Moscow
K. Kapitanova
G. N. Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology, Federal Service on Surveillance for Consumer Rights Protection and Human Well-Being
Email: toptyginaanna@rambler.ru
Россия, Moscow
E. Semikina
National Medical Research Center for Children’s Health, Ministry of Health of the Russian Federation
Email: toptyginaanna@rambler.ru
Россия, Moscow
