Detection of Small Subsets of CD4+ Lymphocytes with SmartFlare Nanoprobes


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Resumo

SmartFlare technology allows detection of mRNA in single living cells. We studied the possibility of using SmartFlare nanoprobes for detection of small subsets of CD4+ lymphocytes. It was found that SmartFlare allows detection of transcriptional master regulators of major CD4+T helper subsets in living human lymphocytes. Nanoprobes labeled with Cy5 fluorophore were better detected by flow cytometry than nanoprobes labeled with Cy3. Appropriate time of lymphocyte incubation with SmartFlare probes was 24 h.

Sobre autores

A. Toptygina

G. N. Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology, Federal Service on Surveillance for Consumer Rights Protection and Human Well-Being

Autor responsável pela correspondência
Email: toptyginaanna@rambler.ru
Rússia, Moscow

R. Zakirov

National Medical Research Center for Children’s Health, Ministry of Health of the Russian Federation

Email: toptyginaanna@rambler.ru
Rússia, Moscow

K. Kapitanova

G. N. Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology, Federal Service on Surveillance for Consumer Rights Protection and Human Well-Being

Email: toptyginaanna@rambler.ru
Rússia, Moscow

E. Semikina

National Medical Research Center for Children’s Health, Ministry of Health of the Russian Federation

Email: toptyginaanna@rambler.ru
Rússia, Moscow


Declaração de direitos autorais © Springer Science+Business Media, LLC, part of Springer Nature, 2019

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