Stably Fluorescent Cell Line of Human Ovarian Epithelial Cancer Cells SK-OV-3ip-red
- Authors: Konovalova E.V.1, Shulga A.A.1, Chumakov S.P.1, Khodarovich Y.M.1, Woo E.2, Deev S.M.1
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Affiliations:
- M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Korea Research Institute of Bioscience and Biotechnology (KRIBB)
- Issue: Vol 164, No 1 (2017)
- Pages: 99-101
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/239392
- DOI: https://doi.org/10.1007/s10517-017-3933-5
- ID: 239392
Cite item
Abstract
Stable red fluorescing line of human ovarian epithelial cancer cells SK-OV-3ip-red was generated expressing gene coding for protein TurboFP635 (Katushka) fluorescing in the far-red spectrum region with excitation and emission peaks at 588 and 635 nm, respectively. Fluorescence of SK-OV-3ip-red line remained high during long-term cell culturing and after cryogenic freezing. The obtained cell line SK-OV-3ip-red can serve a basis for a model of a scattered tumor with numerous/extended metastases and used both for testing anticancer drugs inhibiting metastasis growth and for non-invasive monitoring of the growth dynamics with high precision.
About the authors
E. V. Konovalova
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: deyev@mx.ibch.ru
Russian Federation, Moscow
A. A. Shulga
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: deyev@mx.ibch.ru
Russian Federation, Moscow
S. P. Chumakov
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: deyev@mx.ibch.ru
Russian Federation, Moscow
Yu. M. Khodarovich
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: deyev@mx.ibch.ru
Russian Federation, Moscow
Eui-Jeon Woo
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
Email: deyev@mx.ibch.ru
Korea, Republic of, Daejeon
S. M. Deev
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Author for correspondence.
Email: deyev@mx.ibch.ru
Russian Federation, Moscow