Stably Fluorescent Cell Line of Human Ovarian Epithelial Cancer Cells SK-OV-3ip-red

E. V. Konovalova; A. A. Shulga; S. P. Chumakov; Yu. M. Khodarovich; Eui-Jeon Woo; S. M. Deev

doi:10.1007/s10517-017-3933-5

Stably Fluorescent Cell Line of Human Ovarian Epithelial Cancer Cells SK-OV-3ip-red

Authors: Konovalova E.V.¹, Shulga A.A.¹, Chumakov S.P.¹, Khodarovich Y.M.¹, Woo E.², Deev S.M.¹
Affiliations:
1. M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
2. Korea Research Institute of Bioscience and Biotechnology (KRIBB)
Issue: Vol 164, No 1 (2017)
Pages: 99-101
Section: Article
URL: https://journals.rcsi.science/0007-4888/article/view/239392
DOI: https://doi.org/10.1007/s10517-017-3933-5
ID: 239392

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Abstract
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Abstract

Stable red fluorescing line of human ovarian epithelial cancer cells SK-OV-3ip-red was generated expressing gene coding for protein TurboFP635 (Katushka) fluorescing in the far-red spectrum region with excitation and emission peaks at 588 and 635 nm, respectively. Fluorescence of SK-OV-3ip-red line remained high during long-term cell culturing and after cryogenic freezing. The obtained cell line SK-OV-3ip-red can serve a basis for a model of a scattered tumor with numerous/extended metastases and used both for testing anticancer drugs inhibiting metastasis growth and for non-invasive monitoring of the growth dynamics with high precision.

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Stably Fluorescent Cell Line of Human Ovarian Epithelial Cancer Cells SK-OV-3ip-red

Full Text

Abstract

About the authors

E. V. Konovalova

A. A. Shulga

S. P. Chumakov

Yu. M. Khodarovich

Eui-Jeon Woo

S. M. Deev

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