Lipoxygenase Inhibitors Nordihydroguaiaretic Acid and Fungus Lecanicillum lecanii Extract Induce Death of Lymphoid Leukemia Cells
- Authors: Bibikova M.V.1, Spiridonova N.A.1, Korystova A.F.2, Kublik L.N.2, Levitman M.K.2, Shaposhnikova V.V.2, Korystov Y.N.2
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Affiliations:
- VIORIN Company
- Institute of Theoretical and Experimental Biophysics, Russian Academy of Science
- Issue: Vol 163, No 3 (2017)
- Pages: 330-333
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/238862
- DOI: https://doi.org/10.1007/s10517-017-3796-9
- ID: 238862
Cite item
Abstract
We studied the effect of lipoxygenase inhibitors nordihydroguaiaretic acid (NDGA) and fungus Lecanicillum lecanii extract on lymphatic leukemia P388 cells. The cells grown in the abdominal cavity of DBA2 mice for 7 days were transferred into a nutrient medium. The effect of lipoxygenase inhibitors was evaluated by changes in cell number, trypan blue staining, nucleus damage, and changes in cell distribution by DNA content after 22-h incubation. NDGA and fungus extract induced apoptotic death of lymphatic leukemia cells, which was seen from nucleus damage and reduced DNA content in cells. IC50 for NDGA and fungus extract was 0.66 and 5.5 μg/ml, respectively.
About the authors
M. V. Bibikova
VIORIN Company
Email: ykorostov@rambler.ru
Russian Federation, Moscow
N. A. Spiridonova
VIORIN Company
Email: ykorostov@rambler.ru
Russian Federation, Moscow
A. F. Korystova
Institute of Theoretical and Experimental Biophysics, Russian Academy of Science
Email: ykorostov@rambler.ru
Russian Federation, Pushchino, Moscow region
L. N. Kublik
Institute of Theoretical and Experimental Biophysics, Russian Academy of Science
Email: ykorostov@rambler.ru
Russian Federation, Pushchino, Moscow region
M. Kh. Levitman
Institute of Theoretical and Experimental Biophysics, Russian Academy of Science
Email: ykorostov@rambler.ru
Russian Federation, Pushchino, Moscow region
V. V. Shaposhnikova
Institute of Theoretical and Experimental Biophysics, Russian Academy of Science
Email: ykorostov@rambler.ru
Russian Federation, Pushchino, Moscow region
Yu. N. Korystov
Institute of Theoretical and Experimental Biophysics, Russian Academy of Science
Author for correspondence.
Email: ykorostov@rambler.ru
Russian Federation, Pushchino, Moscow region