Regulatory Effects of Urokinase on Mesenchymal Stromal Cell Migration, Proliferation, and Matrix Metalloproteinase Secretion
- Authors: Beloglazova I.B.1, Zubkova E.S.1, Tsokolaeva Z.I.1, Stafeev Y.S.1, Dergilev K.V.1, Ratner E.I.1, Shestakova M.V.2, Sukhareva O.Y.2, Parfenova E.V.1, Men’shikov M.Y.1
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Affiliations:
- Russian Cardiology Research and Production Complex (RCRPC)
- Endocrinology Research Centre, Ministry of Health of the Russian Federation
- Issue: Vol 161, No 6 (2016)
- Pages: 775-778
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/237765
- DOI: https://doi.org/10.1007/s10517-016-3507-y
- ID: 237765
Cite item
Abstract
We studied the effect of urokinase, its recombinant forms, and domain fragments on migration and proliferation of adipose tissue mesenchymal stromal cells (MSCs) and MMP secretion by these cells. Urokinase, but not its recombinant forms, slightly induced directed migration of MSCs. Spontaneous migration of MSCs increased under the action of urokinase or its isolated kringle domain. Migration induced by platelet-derived growth factor was inhibited by proteolytically inactive form of urokinase, the kringle domain, and blocking antibody to urokinase receptor. Urokinase, its proteolytically inactive form, and kringle domain produced no effect on MSC proliferation. In contrast to platelet-derived growth factor, all urokinase forms induced secretion of MMP-9 by MSCs.
About the authors
I. B. Beloglazova
Russian Cardiology Research and Production Complex (RCRPC)
Author for correspondence.
Email: irene.beloglazova@gmail.com
Russian Federation, Moscow
E. S. Zubkova
Russian Cardiology Research and Production Complex (RCRPC)
Email: irene.beloglazova@gmail.com
Russian Federation, Moscow
Z. I. Tsokolaeva
Russian Cardiology Research and Production Complex (RCRPC)
Email: irene.beloglazova@gmail.com
Russian Federation, Moscow
Yu. S. Stafeev
Russian Cardiology Research and Production Complex (RCRPC)
Email: irene.beloglazova@gmail.com
Russian Federation, Moscow
K. V. Dergilev
Russian Cardiology Research and Production Complex (RCRPC)
Email: irene.beloglazova@gmail.com
Russian Federation, Moscow
E. I. Ratner
Russian Cardiology Research and Production Complex (RCRPC)
Email: irene.beloglazova@gmail.com
Russian Federation, Moscow
M. V. Shestakova
Endocrinology Research Centre, Ministry of Health of the Russian Federation
Email: irene.beloglazova@gmail.com
Russian Federation, Moscow
O. Yu. Sukhareva
Endocrinology Research Centre, Ministry of Health of the Russian Federation
Email: irene.beloglazova@gmail.com
Russian Federation, Moscow
E. V. Parfenova
Russian Cardiology Research and Production Complex (RCRPC)
Email: irene.beloglazova@gmail.com
Russian Federation, Moscow
M. Yu. Men’shikov
Russian Cardiology Research and Production Complex (RCRPC)
Email: irene.beloglazova@gmail.com
Russian Federation, Moscow