Regulatory Effects of Urokinase on Mesenchymal Stromal Cell Migration, Proliferation, and Matrix Metalloproteinase Secretion
- Авторы: Beloglazova I.1, Zubkova E.1, Tsokolaeva Z.1, Stafeev Y.1, Dergilev K.1, Ratner E.1, Shestakova M.2, Sukhareva O.2, Parfenova E.1, Men’shikov M.1
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Учреждения:
- Russian Cardiology Research and Production Complex (RCRPC)
- Endocrinology Research Centre, Ministry of Health of the Russian Federation
- Выпуск: Том 161, № 6 (2016)
- Страницы: 775-778
- Раздел: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/237765
- DOI: https://doi.org/10.1007/s10517-016-3507-y
- ID: 237765
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Аннотация
We studied the effect of urokinase, its recombinant forms, and domain fragments on migration and proliferation of adipose tissue mesenchymal stromal cells (MSCs) and MMP secretion by these cells. Urokinase, but not its recombinant forms, slightly induced directed migration of MSCs. Spontaneous migration of MSCs increased under the action of urokinase or its isolated kringle domain. Migration induced by platelet-derived growth factor was inhibited by proteolytically inactive form of urokinase, the kringle domain, and blocking antibody to urokinase receptor. Urokinase, its proteolytically inactive form, and kringle domain produced no effect on MSC proliferation. In contrast to platelet-derived growth factor, all urokinase forms induced secretion of MMP-9 by MSCs.
Об авторах
I. Beloglazova
Russian Cardiology Research and Production Complex (RCRPC)
Автор, ответственный за переписку.
Email: irene.beloglazova@gmail.com
Россия, Moscow
E. Zubkova
Russian Cardiology Research and Production Complex (RCRPC)
Email: irene.beloglazova@gmail.com
Россия, Moscow
Z. Tsokolaeva
Russian Cardiology Research and Production Complex (RCRPC)
Email: irene.beloglazova@gmail.com
Россия, Moscow
Yu. Stafeev
Russian Cardiology Research and Production Complex (RCRPC)
Email: irene.beloglazova@gmail.com
Россия, Moscow
K. Dergilev
Russian Cardiology Research and Production Complex (RCRPC)
Email: irene.beloglazova@gmail.com
Россия, Moscow
E. Ratner
Russian Cardiology Research and Production Complex (RCRPC)
Email: irene.beloglazova@gmail.com
Россия, Moscow
M. Shestakova
Endocrinology Research Centre, Ministry of Health of the Russian Federation
Email: irene.beloglazova@gmail.com
Россия, Moscow
O. Sukhareva
Endocrinology Research Centre, Ministry of Health of the Russian Federation
Email: irene.beloglazova@gmail.com
Россия, Moscow
E. Parfenova
Russian Cardiology Research and Production Complex (RCRPC)
Email: irene.beloglazova@gmail.com
Россия, Moscow
M. Men’shikov
Russian Cardiology Research and Production Complex (RCRPC)
Email: irene.beloglazova@gmail.com
Россия, Moscow