Email this article Quantitative and Qualitative Characterization of Phagocytic Activity of Macrophages of Bone Marrow and Fetal Origin
- Authors: Lokhonina A.V.1,2, Makarov A.V.1,3, Elchaninov A.V.1,2, Arutyunyan I.V.1,4, Shmakova T.V.4, Grinberg M.V.2, Usman N.Y.1, Surovtsev V.V.2, Chernikov V.P.4, Fatkhudinov T.K.1,2
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Affiliations:
- V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- Peoples’ Friendship University of Russia
- N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
- Research Institute of Human Morphology, Research Institute of Human Morphology
- Issue: Vol 167, No 1 (2019)
- Pages: 154-158
- Section: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/241535
- DOI: https://doi.org/10.1007/s10517-019-04481-5
- ID: 241535
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Abstract
We compared phagocytic activity of macrophages of monocyte origin and Kupffer cells under the influence of M1 and M2 inducers and without activation. Cultures of monocyte-derived macrophages and Kupffer cells were characterized by intensive expression of CD68 that was not affected by activation factors. At the same time, these cultures demonstrated different dynamics of phagocytic activity. Monocyte-derived macrophages initially had more pronounced absorption capacity that gradually increased during the experiment. Kupffer cells were characterized by abrupt fluctuations of phagocytic activity: sharp growth and rapid saturation. Despite these differences, the endosomes produced by monocyte-derived macrophages and Kupffer cells had similar degrees of maturity.
About the authors
A. V. Lokhonina
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation; Peoples’ Friendship University of Russia
Email: elchandrey@yandex.ru
Russian Federation, Moscow; Moscow
A. V. Makarov
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation; N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: elchandrey@yandex.ru
Russian Federation, Moscow; Moscow
A. V. Elchaninov
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation; Peoples’ Friendship University of Russia
Author for correspondence.
Email: elchandrey@yandex.ru
Russian Federation, Moscow; Moscow
I. V. Arutyunyan
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation; Research Institute of Human Morphology, Research Institute of Human Morphology
Email: elchandrey@yandex.ru
Russian Federation, Moscow; Moscow
T. V. Shmakova
Research Institute of Human Morphology, Research Institute of Human Morphology
Email: elchandrey@yandex.ru
Russian Federation, Moscow
M. V. Grinberg
Peoples’ Friendship University of Russia
Email: elchandrey@yandex.ru
Russian Federation, Moscow
N. Yu. Usman
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: elchandrey@yandex.ru
Russian Federation, Moscow
V. V. Surovtsev
Peoples’ Friendship University of Russia
Email: elchandrey@yandex.ru
Russian Federation, Moscow
V. P. Chernikov
Research Institute of Human Morphology, Research Institute of Human Morphology
Email: elchandrey@yandex.ru
Russian Federation, Moscow
T. Kh. Fatkhudinov
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation; Peoples’ Friendship University of Russia
Email: elchandrey@yandex.ru
Russian Federation, Moscow; Moscow
