Email this article Assessment of Lactate Production and Proteoglycans Synthesis by the Intact and Degenerated Intervertebral Disc Cells under the Influence of Activated Macrophages: an In Vitro Study
- Authors: Byvaltsev V.A.1,2,3, Kolesnikov S.I.4,5, Bardonova L.A.1,6, Belykh E.G1,6, Korytov L.I.1, Giers M.B.7, Preul M.C.6
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Affiliations:
- Irkutsk State Medical University
- Irkutsk Research Center of Surgery and Traumatology
- Railroad Clinical Hospital at Irkutsk-Passenger Station, Russian Railways Company
- Research Center for Problems of Family Health and Human Reproduction
- M. V. Lomonosov Moscow State University
- Barrow Neurological Institute
- Oregon State University
- Issue: Vol 166, No 1 (2018)
- Pages: 170-173
- Section: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/240888
- DOI: https://doi.org/10.1007/s10517-018-4307-3
- ID: 240888
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Abstract
The effects of proinflammatory cytokines on the secretion of glycosaminoglycans and lactate production by normal and degenerated intervertebral disk cells were studied on the model of their co-culturing with activated macrophage-like cells. It was found that proinflammatory cytokines produced a direct effect on intervertebral disk cells in a 3D culture reducing the rate of glycolysis and synthetic activity of both normal and degenerated cells of annulus fibrosus and nucleus pulposus, which is an important factor in progression of intervertebral disk degeneration.
About the authors
V. A. Byvaltsev
Irkutsk State Medical University; Irkutsk Research Center of Surgery and Traumatology; Railroad Clinical Hospital at Irkutsk-Passenger Station, Russian Railways Company
Author for correspondence.
Email: byval75vadim@yandex.ru
Russian Federation, Irkutsk; Irkutsk; Irkutsk
S. I. Kolesnikov
Research Center for Problems of Family Health and Human Reproduction; M. V. Lomonosov Moscow State University
Email: byval75vadim@yandex.ru
Russian Federation, Irkutsk; Moscow
L. A. Bardonova
Irkutsk State Medical University; Barrow Neurological Institute
Email: byval75vadim@yandex.ru
Russian Federation, Irkutsk; Phoenix, AZ
E. G Belykh
Irkutsk State Medical University; Barrow Neurological Institute
Email: byval75vadim@yandex.ru
Russian Federation, Irkutsk; Phoenix, AZ
L. I. Korytov
Irkutsk State Medical University
Email: byval75vadim@yandex.ru
Russian Federation, Irkutsk
M. B. Giers
Oregon State University
Email: byval75vadim@yandex.ru
United States, Corvallis, OR
M. C. Preul
Barrow Neurological Institute
Email: byval75vadim@yandex.ru
United States, Phoenix, AZ
