Email this article In Vitro Model for Studying of the Role of IGFBP6 Gene in Breast Cancer Metastasizing
- Authors: Nikulin S.V.1,2, Raigorodskaya M.P.1, Poloznikov A.A.1, Zakharova G.S.1, Schumacher U.3, Wicklein D.3, Stürken C.3, Riecken K.3, Fomicheva K.A.4, Alekseev B.Y.4, Shkurnikov M.Y.4
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Affiliations:
- BioClinicum Center
- Moscow Institute of Physics and Technology (MIPT)
- University Medical Center Hamburg-Eppendorf
- P. A. Hertsen Moscow Oncology Research Center, Branch of Branch of National Medical Research Radiology Center of the Ministry of Health of the Russian Federation
- Issue: Vol 164, No 5 (2018)
- Pages: 688-692
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/239856
- DOI: https://doi.org/10.1007/s10517-018-4060-7
- ID: 239856
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Abstract
IGFBP6 gene plays an important role in the pathogenesis of breast cancer. In this work, we performed knockdown of IGFBP6 gene in MDA-MB-231 cells and obtained a stable cell line. Knockdown of IGFBP6 gene was confirmed by the real-time PCR. The influence of IGFBP6 gene on migration and proliferation of breast cancer cells was studied. Knockdown of IGFBP6 gene reduced migration activity of MDA-MB-231 cells and increased their proliferation rate. This in vitro cell model can be used for the further analysis of the role of IGFBP6 gene in the pathogenesis of breast cancer.
About the authors
S. V. Nikulin
BioClinicum Center; Moscow Institute of Physics and Technology (MIPT)
Email: mshkurnikov@gmail.com
Russian Federation, Moscow; Moscow
M. P. Raigorodskaya
BioClinicum Center
Email: mshkurnikov@gmail.com
Russian Federation, Moscow
A. A. Poloznikov
BioClinicum Center
Email: mshkurnikov@gmail.com
Russian Federation, Moscow
G. S. Zakharova
BioClinicum Center
Email: mshkurnikov@gmail.com
Russian Federation, Moscow
U. Schumacher
University Medical Center Hamburg-Eppendorf
Email: mshkurnikov@gmail.com
Germany, Hamburg
D. Wicklein
University Medical Center Hamburg-Eppendorf
Email: mshkurnikov@gmail.com
Germany, Hamburg
C. Stürken
University Medical Center Hamburg-Eppendorf
Email: mshkurnikov@gmail.com
Germany, Hamburg
K. Riecken
University Medical Center Hamburg-Eppendorf
Email: mshkurnikov@gmail.com
Germany, Hamburg
K. A. Fomicheva
P. A. Hertsen Moscow Oncology Research Center, Branch of Branch of National Medical Research Radiology Center of the Ministry of Health of the Russian Federation
Email: mshkurnikov@gmail.com
Russian Federation, Moscow
B. Ya. Alekseev
P. A. Hertsen Moscow Oncology Research Center, Branch of Branch of National Medical Research Radiology Center of the Ministry of Health of the Russian Federation
Email: mshkurnikov@gmail.com
Russian Federation, Moscow
M. Yu. Shkurnikov
P. A. Hertsen Moscow Oncology Research Center, Branch of Branch of National Medical Research Radiology Center of the Ministry of Health of the Russian Federation
Author for correspondence.
Email: mshkurnikov@gmail.com
Russian Federation, Moscow
