Melatonin—Aluminum Oxide—Polymethylsiloxane Complex on Apoptosis of Liver Cells in a Model of Obesity and Type 2 Diabetes Mellitus
- Authors: Michurina S.V.1, Ischenko I.Y.1, Arkhipov S.A.1, Klimontov V.V.1, Cherepanova M.A.1, Korolev M.A.1, Rachkovskaya L.N.1, Zav’yalov E.L.2, Konenkov V.I.1
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Affiliations:
- Research Institute of Clinical and Experimental Lymphology, Siberian Division of the Russian Academy of Science
- Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Science
- Issue: Vol 164, No 2 (2017)
- Pages: 165-169
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/239444
- DOI: https://doi.org/10.1007/s10517-017-3949-x
- ID: 239444
Cite item
Abstract
We studied the effects of a melatonin—aluminum oxide—polymethylsiloxane complex (complex M) on the expression of apoptosis regulators Bcl-2 and Bad in the liver of homozygous db/db BKS.Cg-Dock7m+/+Leprdb/J mice with obesity and type 2 diabetes. Complex M or placebo was administered daily through the gastric tube during weeks 8-16 of life. In mice with type 2 diabetes mellitus receiving placebo, enhanced immunohistochemical reactions for proapoptotic Bad protein and weak response for anti-apoptotic Bcl-2 protein were observed. Administration of complex M shifted the ratio of apoptosis regulators: the area of Bcl-2 expression significantly increased and against the background of reduced Bad expression area. These findings attest to antiapoptotic effect of complex M in the liver on the model of type 2 diabetes mellitus.
About the authors
S. V. Michurina
Research Institute of Clinical and Experimental Lymphology, Siberian Division of the Russian Academy of Science
Author for correspondence.
Email: s.michurina@ngs.ru
Russian Federation, Novosibirsk
I. Yu. Ischenko
Research Institute of Clinical and Experimental Lymphology, Siberian Division of the Russian Academy of Science
Email: s.michurina@ngs.ru
Russian Federation, Novosibirsk
S. A. Arkhipov
Research Institute of Clinical and Experimental Lymphology, Siberian Division of the Russian Academy of Science
Email: s.michurina@ngs.ru
Russian Federation, Novosibirsk
V. V. Klimontov
Research Institute of Clinical and Experimental Lymphology, Siberian Division of the Russian Academy of Science
Email: s.michurina@ngs.ru
Russian Federation, Novosibirsk
M. A. Cherepanova
Research Institute of Clinical and Experimental Lymphology, Siberian Division of the Russian Academy of Science
Email: s.michurina@ngs.ru
Russian Federation, Novosibirsk
M. A. Korolev
Research Institute of Clinical and Experimental Lymphology, Siberian Division of the Russian Academy of Science
Email: s.michurina@ngs.ru
Russian Federation, Novosibirsk
L. N. Rachkovskaya
Research Institute of Clinical and Experimental Lymphology, Siberian Division of the Russian Academy of Science
Email: s.michurina@ngs.ru
Russian Federation, Novosibirsk
E. L. Zav’yalov
Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Science
Email: s.michurina@ngs.ru
Russian Federation, Novosibirsk
V. I. Konenkov
Research Institute of Clinical and Experimental Lymphology, Siberian Division of the Russian Academy of Science
Email: s.michurina@ngs.ru
Russian Federation, Novosibirsk