Email this article Recombinant Human Bone Morphogenetic Protein-2 (rhBMP-2) with Additional Protein Domain Synthesized in E. coli: In Vivo Osteoinductivity in Experimental Models on Small and Large Laboratory Animals
- Authors: Bartov M.S.1, Gromov A.V.1, Manskih V.N.1,2, Makarova E.B.3, Rubshtein A.P.4, Poponova M.S.1, Savina D.M.1, Savin K.S.1, Nikitin K.E.1, Grunina T.M.1, Boksha I.S.1, Orlova P.A.1, Krivozubov M.S.1, Subbotina M.E.1,5, Lunin V.G.1,5, Karyagina A.S.1,2,5, Gintsburg A.L.1
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Affiliations:
- N. F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation
- A. N. Belozersky Research Institute of Physicochemical Biology, M. V. Lomonosov Moscow State University
- Center of Specialized Types of Medical Care, V.D. Chaklin Ural Institute of Traumatology and Orthopedics
- M. N. Mikheev Institute of Metal Physics, Ural Division of the Russian Academy of Sciences
- All-Russian Research Institute of Agricultural Biotechnology
- Issue: Vol 164, No 2 (2017)
- Pages: 148-151
- Section: Biophysics and Biochemistry
- URL: https://journals.rcsi.science/0007-4888/article/view/239433
- DOI: https://doi.org/10.1007/s10517-017-3945-1
- ID: 239433
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Abstract
Recombinant human bone morphogenetic protein-2 with an additional s-tag domain (s-tag-BMP-2) synthesized in E. coli is characterized by higher solubility and activity than the protein without additional s-tag domain, which increases the yield during purification and simplifies protein introduction into the osteoplastic materials. The high osteoinductivity of the demineralized bone matrix with s-tag-BMP-2 was shown on the model of regeneration of cranial defects of a critical size in mice and on the model of implantation of porous titanium matrix into defects of femoral and tibial bones in rabbits.
About the authors
M. S. Bartov
N. F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation
Author for correspondence.
Email: mike.bartov@gmail.com
Russian Federation, Moscow
A. V. Gromov
N. F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation
Email: mike.bartov@gmail.com
Russian Federation, Moscow
V. N. Manskih
N. F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation; A. N. Belozersky Research Institute of Physicochemical Biology, M. V. Lomonosov Moscow State University
Email: mike.bartov@gmail.com
Russian Federation, Moscow; Moscow
E. B. Makarova
Center of Specialized Types of Medical Care, V.D. Chaklin Ural Institute of Traumatology and Orthopedics
Email: mike.bartov@gmail.com
Russian Federation, Ekaterinburg
A. P. Rubshtein
M. N. Mikheev Institute of Metal Physics, Ural Division of the Russian Academy of Sciences
Email: mike.bartov@gmail.com
Russian Federation, Ekaterinburg
M. S. Poponova
N. F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation
Email: mike.bartov@gmail.com
Russian Federation, Moscow
D. M. Savina
N. F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation
Email: mike.bartov@gmail.com
Russian Federation, Moscow
K. S. Savin
N. F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation
Email: mike.bartov@gmail.com
Russian Federation, Moscow
K. E. Nikitin
N. F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation
Email: mike.bartov@gmail.com
Russian Federation, Moscow
T. M. Grunina
N. F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation
Email: mike.bartov@gmail.com
Russian Federation, Moscow
I. S. Boksha
N. F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation
Email: mike.bartov@gmail.com
Russian Federation, Moscow
P. A. Orlova
N. F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation
Email: mike.bartov@gmail.com
Russian Federation, Moscow
M. S. Krivozubov
N. F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation
Email: mike.bartov@gmail.com
Russian Federation, Moscow
M. E. Subbotina
N. F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation; All-Russian Research Institute of Agricultural Biotechnology
Email: mike.bartov@gmail.com
Russian Federation, Moscow; Moscow
V. G. Lunin
N. F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation; All-Russian Research Institute of Agricultural Biotechnology
Email: mike.bartov@gmail.com
Russian Federation, Moscow; Moscow
A. S. Karyagina
N. F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation; A. N. Belozersky Research Institute of Physicochemical Biology, M. V. Lomonosov Moscow State University; All-Russian Research Institute of Agricultural Biotechnology
Email: mike.bartov@gmail.com
Russian Federation, Moscow; Moscow; Moscow
A. L. Gintsburg
N. F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of the Russian Federation
Email: mike.bartov@gmail.com
Russian Federation, Moscow
