Email this article Preparation of Recombinant Serpin from Red King Crab Paralithodes сamtschaticus for Biomedical Research Purposes
- Authors: Kostin N.N.1,2, Ponomarenko N.A.1, Isaev V.A.3, Rudenskaya G.N.1, Bobik T.V.1, Gabibov A.G.1,2, Smirnov I.V.1,2
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Affiliations:
- M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences
- Faculty of Chemistry, M. V. Lomonosov Moscow State University
- TRINITA Research and Production Company
- Issue: Vol 163, No 2 (2017)
- Pages: 210-213
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/238758
- DOI: https://doi.org/10.1007/s10517-017-3768-0
- ID: 238758
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Abstract
Genetic constructs with different leader sequences for intra- and extracellular expression of the target protein were generated and an original method for effective selection of clones with maximum expression was developed. For intracellular expression in the Pichia pastoris system, seprin content in cells was 6 mg/liter.
About the authors
N. N. Kostin
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences; Faculty of Chemistry, M. V. Lomonosov Moscow State University
Email: ivansmr@inbox.ru
Russian Federation, Moscow; Moscow
N. A. Ponomarenko
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences
Email: ivansmr@inbox.ru
Russian Federation, Moscow
V. A. Isaev
TRINITA Research and Production Company
Email: ivansmr@inbox.ru
Russian Federation, Moscow Region
G. N. Rudenskaya
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences
Email: ivansmr@inbox.ru
Russian Federation, Moscow
T. V. Bobik
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences
Email: ivansmr@inbox.ru
Russian Federation, Moscow
A. G. Gabibov
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences; Faculty of Chemistry, M. V. Lomonosov Moscow State University
Email: ivansmr@inbox.ru
Russian Federation, Moscow; Moscow
I. V. Smirnov
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences; Faculty of Chemistry, M. V. Lomonosov Moscow State University
Author for correspondence.
Email: ivansmr@inbox.ru
Russian Federation, Moscow; Moscow
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