Мақаланы E-mail арқылы жіберу Preparation of Recombinant Serpin from Red King Crab Paralithodes сamtschaticus for Biomedical Research Purposes
- Авторлар: Kostin N.N.1,2, Ponomarenko N.A.1, Isaev V.A.3, Rudenskaya G.N.1, Bobik T.V.1, Gabibov A.G.1,2, Smirnov I.V.1,2
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Мекемелер:
- M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences
- Faculty of Chemistry, M. V. Lomonosov Moscow State University
- TRINITA Research and Production Company
- Шығарылым: Том 163, № 2 (2017)
- Беттер: 210-213
- Бөлім: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/238758
- DOI: https://doi.org/10.1007/s10517-017-3768-0
- ID: 238758
Дәйексөз келтіру
Ашық рұқсат
Рұқсат берілді
Тек жазылушылар үшін
Аннотация
Genetic constructs with different leader sequences for intra- and extracellular expression of the target protein were generated and an original method for effective selection of clones with maximum expression was developed. For intracellular expression in the Pichia pastoris system, seprin content in cells was 6 mg/liter.
Авторлар туралы
N. Kostin
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences; Faculty of Chemistry, M. V. Lomonosov Moscow State University
Email: ivansmr@inbox.ru
Ресей, Moscow; Moscow
N. Ponomarenko
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences
Email: ivansmr@inbox.ru
Ресей, Moscow
V. Isaev
TRINITA Research and Production Company
Email: ivansmr@inbox.ru
Ресей, Moscow Region
G. Rudenskaya
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences
Email: ivansmr@inbox.ru
Ресей, Moscow
T. Bobik
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences
Email: ivansmr@inbox.ru
Ресей, Moscow
A. Gabibov
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences; Faculty of Chemistry, M. V. Lomonosov Moscow State University
Email: ivansmr@inbox.ru
Ресей, Moscow; Moscow
I. Smirnov
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences; Faculty of Chemistry, M. V. Lomonosov Moscow State University
Хат алмасуға жауапты Автор.
Email: ivansmr@inbox.ru
Ресей, Moscow; Moscow
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