电邮这篇文章 Preparation of Recombinant Serpin from Red King Crab Paralithodes сamtschaticus for Biomedical Research Purposes
- 作者: Kostin N.N.1,2, Ponomarenko N.A.1, Isaev V.A.3, Rudenskaya G.N.1, Bobik T.V.1, Gabibov A.G.1,2, Smirnov I.V.1,2
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隶属关系:
- M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences
- Faculty of Chemistry, M. V. Lomonosov Moscow State University
- TRINITA Research and Production Company
- 期: 卷 163, 编号 2 (2017)
- 页面: 210-213
- 栏目: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/238758
- DOI: https://doi.org/10.1007/s10517-017-3768-0
- ID: 238758
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详细
Genetic constructs with different leader sequences for intra- and extracellular expression of the target protein were generated and an original method for effective selection of clones with maximum expression was developed. For intracellular expression in the Pichia pastoris system, seprin content in cells was 6 mg/liter.
作者简介
N. Kostin
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences; Faculty of Chemistry, M. V. Lomonosov Moscow State University
Email: ivansmr@inbox.ru
俄罗斯联邦, Moscow; Moscow
N. Ponomarenko
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences
Email: ivansmr@inbox.ru
俄罗斯联邦, Moscow
V. Isaev
TRINITA Research and Production Company
Email: ivansmr@inbox.ru
俄罗斯联邦, Moscow Region
G. Rudenskaya
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences
Email: ivansmr@inbox.ru
俄罗斯联邦, Moscow
T. Bobik
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences
Email: ivansmr@inbox.ru
俄罗斯联邦, Moscow
A. Gabibov
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences; Faculty of Chemistry, M. V. Lomonosov Moscow State University
Email: ivansmr@inbox.ru
俄罗斯联邦, Moscow; Moscow
I. Smirnov
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences; Faculty of Chemistry, M. V. Lomonosov Moscow State University
编辑信件的主要联系方式.
Email: ivansmr@inbox.ru
俄罗斯联邦, Moscow; Moscow
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