电邮这篇文章 The Effect of Polyoxyethylene–Polyoxypropylene Triblock Copolymers on the Loading Degree of Poly-(Lactic-co-Glycolic Acid) Copolymer-Based Microparticles Containing Chlorin e6 and Ethidium Bromide in Mesenchymal Stem Cells
- 作者: Temnov A.A.1,2,3, Sklifas A.N.1, Kukushkin N.I.1, Krechetov S.P.2, Gorina E.V.2, Astrelina T.A.3, Usupzhanova D.Y.3, Suchkova Y.B.3, Kobzeva I.V.3, Samoilov A.S.3
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隶属关系:
- Institute of Cell Biophysics, Federal Research Center Pushchino Scientific Center of Biological Investigations, Russian Academy of Sciences
- Moscow Institute of Physics and Technology (State University)
- Burnasyan Federal Medical Biophysical Center, Federal Medical Biological Agency of Russia
- 期: 卷 64, 编号 2 (2019)
- 页面: 224-232
- 栏目: Cell Biophysics
- URL: https://journals.rcsi.science/0006-3509/article/view/152899
- DOI: https://doi.org/10.1134/S0006350919020179
- ID: 152899
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详细
The effect of different polyoxyethylene–polyoxypropylene triblock copolymers, their concentration, and mode of action on the loading of poly-(lactic-co-glycolic acid) copolymer-based microparticles containing such medicinal agents as radachlorin (chlorin e6) or ethidium bromide in mesenchymal stem cells was studied. It has been shown that medicinal agents encapsulated inside microparticles affect the loading of these particles in the cytoplasm of mesenchymal stem cells. The number of cells that absorbed the particles with chlorin e6 is approximately two times lower than that in the experiments with ethidium bromide. It has been shown that pretreatment of microparticles with triblock copolymers is more efficient for loading them in cells compared with simultaneous introduction of triblock copolymers and particles into the culture medium. Treatment of ethidium bromide-containing microparticles with triblock copolymers is not efficient for their loading in mesenchymal stem cells compared to the control. The exception is Pluronic 123; when particles are treated with it at concentrations of 1 and 2%, the loading of particles in cells increases compared to the control by factors of approximately 11 and 5, respectively. For particles with chlorin e6, their pretreatment with triblock copolymers at a concentration of 4% is most efficient; the loading of the pretreated particles in cells is increased by factors of approximately 3 to 11.
作者简介
A. Temnov
Institute of Cell Biophysics, Federal Research Center Pushchino Scientific Center of Biological Investigations, Russian Academy of Sciences; Moscow Institute of Physics and Technology (State University); Burnasyan Federal Medical Biophysical Center, Federal Medical Biological Agency of Russia
Email: nikukushkin@mail.ru
俄罗斯联邦, Pushchino, Moscow oblast, 142290; Dolgoprudny, Moscow oblast, 141701; Moscow, 123098
A. Sklifas
Institute of Cell Biophysics, Federal Research Center Pushchino Scientific Center of Biological Investigations, Russian Academy of Sciences
Email: nikukushkin@mail.ru
俄罗斯联邦, Pushchino, Moscow oblast, 142290
N. Kukushkin
Institute of Cell Biophysics, Federal Research Center Pushchino Scientific Center of Biological Investigations, Russian Academy of Sciences
编辑信件的主要联系方式.
Email: nikukushkin@mail.ru
俄罗斯联邦, Pushchino, Moscow oblast, 142290
S. Krechetov
Moscow Institute of Physics and Technology (State University)
Email: nikukushkin@mail.ru
俄罗斯联邦, Dolgoprudny, Moscow oblast, 141701
E. Gorina
Moscow Institute of Physics and Technology (State University)
Email: nikukushkin@mail.ru
俄罗斯联邦, Dolgoprudny, Moscow oblast, 141701
T. Astrelina
Burnasyan Federal Medical Biophysical Center, Federal Medical Biological Agency of Russia
Email: nikukushkin@mail.ru
俄罗斯联邦, Moscow, 123098
D. Usupzhanova
Burnasyan Federal Medical Biophysical Center, Federal Medical Biological Agency of Russia
Email: nikukushkin@mail.ru
俄罗斯联邦, Moscow, 123098
Yu. Suchkova
Burnasyan Federal Medical Biophysical Center, Federal Medical Biological Agency of Russia
Email: nikukushkin@mail.ru
俄罗斯联邦, Moscow, 123098
I. Kobzeva
Burnasyan Federal Medical Biophysical Center, Federal Medical Biological Agency of Russia
Email: nikukushkin@mail.ru
俄罗斯联邦, Moscow, 123098
A. Samoilov
Burnasyan Federal Medical Biophysical Center, Federal Medical Biological Agency of Russia
Email: nikukushkin@mail.ru
俄罗斯联邦, Moscow, 123098
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