Email this article Study of heme and globin conformation in fractionated rat erythrocytes by means of raman spectroscopy
- Authors: Yushkov B.G1,2, Zuev M.G3, Brilliant S.A1,2, Vasin A.A3
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Affiliations:
- Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences
- GAUZ SO “Institute for Medical Cell Technologies”
- Institute of Solid State Chemistry, Ural Branch of the Russian Academy of Sciences
- Issue: Vol 68, No 1 (2023)
- Pages: 33-40
- Section: Articles
- URL: https://journals.rcsi.science/0006-3029/article/view/144402
- DOI: https://doi.org/10.31857/S0006302923010040
- EDN: https://elibrary.ru/NZMURQ
- ID: 144402
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Abstract
Conformational changes of heme and globin in fractionated rat erythrocytes have been investigated using Raman spectroscopy. The results obtained show that normal isoforms (common variants) of hemoglobin, which plays a key role in oxygen transport and a protective role against oxidative stress, are dominant (more than 80%) in rat blood. However, heavy chain (3%) and light chain isoforms (11%) formed as a result of protein polymerization or degradation perform important roles in the body because they also function in signal transmission and binding to exogenous ligands.
About the authors
B. G Yushkov
Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences;GAUZ SO “Institute for Medical Cell Technologies”Yekaterinburg, Russia
M. G Zuev
Institute of Solid State Chemistry, Ural Branch of the Russian Academy of SciencesYekaterinburg, Russia
S. A Brilliant
Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences;GAUZ SO “Institute for Medical Cell Technologies”
Email: svetlana.brilliant@bk.ru
Yekaterinburg, Russia
A. A Vasin
Institute of Solid State Chemistry, Ural Branch of the Russian Academy of SciencesYekaterinburg, Russia
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