Construction of Artificial TNF-Binding Proteins Based on the 10th Human Fibronectin Type III Domain Using Bacterial Display
- Авторы: Shingarova L.1, Petrovskaya L.1, Zlobinov A.1,2, Gapizov S.1,2, Kryukova E.1, Birikh K.1, Boldyreva E.1, Yakimov S.1, Dolgikh D.1,2, Kirpichnikov M.1,2
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Учреждения:
- Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
- Lomonosov Moscow State University
- Выпуск: Том 83, № 6 (2018)
- Страницы: 708-716
- Раздел: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151673
- DOI: https://doi.org/10.1134/S0006297918060081
- ID: 151673
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Аннотация
Construction of antibody mimetics on the base of alternative scaffold proteins is a promising strategy for obtaining new products for medicine and biotechnology. The aim of our work was to optimize the cell display system for the 10th human fibronectin type III domain (10Fn3) scaffold protein based on the AT877 autotransporter from Psychrobacter cryohalolentis K5T and to construct new artificial TNF-binding proteins. We obtained a 10Fn3 gene combinatorial library and screened it using the bacterial display method. After expression of the selected 10Fn3 variants in Escherichia coli cells and analysis of their TNF-binding activity, we identified proteins that display high affinity for TNF and characterized their properties.
Об авторах
L. Shingarova
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
Автор, ответственный за переписку.
Email: lshing@ibch.ru
Россия, Moscow, 117997
L. Petrovskaya
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
Email: lshing@ibch.ru
Россия, Moscow, 117997
A. Zlobinov
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry; Lomonosov Moscow State University
Email: lshing@ibch.ru
Россия, Moscow, 117997; Moscow, 119234
S. Gapizov
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry; Lomonosov Moscow State University
Email: lshing@ibch.ru
Россия, Moscow, 117997; Moscow, 119234
E. Kryukova
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
Email: lshing@ibch.ru
Россия, Moscow, 117997
K. Birikh
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
Email: lshing@ibch.ru
Россия, Moscow, 117997
E. Boldyreva
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
Email: lshing@ibch.ru
Россия, Moscow, 117997
S. Yakimov
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
Email: lshing@ibch.ru
Россия, Moscow, 117997
D. Dolgikh
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry; Lomonosov Moscow State University
Email: lshing@ibch.ru
Россия, Moscow, 117997; Moscow, 119234
M. Kirpichnikov
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry; Lomonosov Moscow State University
Email: lshing@ibch.ru
Россия, Moscow, 117997; Moscow, 119234
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