Construction of Artificial TNF-Binding Proteins Based on the 10th Human Fibronectin Type III Domain Using Bacterial Display
- Авторлар: Shingarova L.1, Petrovskaya L.1, Zlobinov A.1,2, Gapizov S.1,2, Kryukova E.1, Birikh K.1, Boldyreva E.1, Yakimov S.1, Dolgikh D.1,2, Kirpichnikov M.1,2
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Мекемелер:
- Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
- Lomonosov Moscow State University
- Шығарылым: Том 83, № 6 (2018)
- Беттер: 708-716
- Бөлім: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151673
- DOI: https://doi.org/10.1134/S0006297918060081
- ID: 151673
Дәйексөз келтіру
Аннотация
Construction of antibody mimetics on the base of alternative scaffold proteins is a promising strategy for obtaining new products for medicine and biotechnology. The aim of our work was to optimize the cell display system for the 10th human fibronectin type III domain (10Fn3) scaffold protein based on the AT877 autotransporter from Psychrobacter cryohalolentis K5T and to construct new artificial TNF-binding proteins. We obtained a 10Fn3 gene combinatorial library and screened it using the bacterial display method. After expression of the selected 10Fn3 variants in Escherichia coli cells and analysis of their TNF-binding activity, we identified proteins that display high affinity for TNF and characterized their properties.
Авторлар туралы
L. Shingarova
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
Хат алмасуға жауапты Автор.
Email: lshing@ibch.ru
Ресей, Moscow, 117997
L. Petrovskaya
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
Email: lshing@ibch.ru
Ресей, Moscow, 117997
A. Zlobinov
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry; Lomonosov Moscow State University
Email: lshing@ibch.ru
Ресей, Moscow, 117997; Moscow, 119234
S. Gapizov
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry; Lomonosov Moscow State University
Email: lshing@ibch.ru
Ресей, Moscow, 117997; Moscow, 119234
E. Kryukova
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
Email: lshing@ibch.ru
Ресей, Moscow, 117997
K. Birikh
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
Email: lshing@ibch.ru
Ресей, Moscow, 117997
E. Boldyreva
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
Email: lshing@ibch.ru
Ресей, Moscow, 117997
S. Yakimov
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
Email: lshing@ibch.ru
Ресей, Moscow, 117997
D. Dolgikh
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry; Lomonosov Moscow State University
Email: lshing@ibch.ru
Ресей, Moscow, 117997; Moscow, 119234
M. Kirpichnikov
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry; Lomonosov Moscow State University
Email: lshing@ibch.ru
Ресей, Moscow, 117997; Moscow, 119234
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