Construction of Artificial TNF-Binding Proteins Based on the 10th Human Fibronectin Type III Domain Using Bacterial Display
- 作者: Shingarova L.1, Petrovskaya L.1, Zlobinov A.1,2, Gapizov S.1,2, Kryukova E.1, Birikh K.1, Boldyreva E.1, Yakimov S.1, Dolgikh D.1,2, Kirpichnikov M.1,2
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隶属关系:
- Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
- Lomonosov Moscow State University
- 期: 卷 83, 编号 6 (2018)
- 页面: 708-716
- 栏目: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151673
- DOI: https://doi.org/10.1134/S0006297918060081
- ID: 151673
如何引用文章
详细
Construction of antibody mimetics on the base of alternative scaffold proteins is a promising strategy for obtaining new products for medicine and biotechnology. The aim of our work was to optimize the cell display system for the 10th human fibronectin type III domain (10Fn3) scaffold protein based on the AT877 autotransporter from Psychrobacter cryohalolentis K5T and to construct new artificial TNF-binding proteins. We obtained a 10Fn3 gene combinatorial library and screened it using the bacterial display method. After expression of the selected 10Fn3 variants in Escherichia coli cells and analysis of their TNF-binding activity, we identified proteins that display high affinity for TNF and characterized their properties.
作者简介
L. Shingarova
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
编辑信件的主要联系方式.
Email: lshing@ibch.ru
俄罗斯联邦, Moscow, 117997
L. Petrovskaya
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
Email: lshing@ibch.ru
俄罗斯联邦, Moscow, 117997
A. Zlobinov
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry; Lomonosov Moscow State University
Email: lshing@ibch.ru
俄罗斯联邦, Moscow, 117997; Moscow, 119234
S. Gapizov
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry; Lomonosov Moscow State University
Email: lshing@ibch.ru
俄罗斯联邦, Moscow, 117997; Moscow, 119234
E. Kryukova
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
Email: lshing@ibch.ru
俄罗斯联邦, Moscow, 117997
K. Birikh
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
Email: lshing@ibch.ru
俄罗斯联邦, Moscow, 117997
E. Boldyreva
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
Email: lshing@ibch.ru
俄罗斯联邦, Moscow, 117997
S. Yakimov
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
Email: lshing@ibch.ru
俄罗斯联邦, Moscow, 117997
D. Dolgikh
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry; Lomonosov Moscow State University
Email: lshing@ibch.ru
俄罗斯联邦, Moscow, 117997; Moscow, 119234
M. Kirpichnikov
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry; Lomonosov Moscow State University
Email: lshing@ibch.ru
俄罗斯联邦, Moscow, 117997; Moscow, 119234