Overview of fusion tags for recombinant proteins
- Autores: Kosobokova E.1, Skrypnik K.1, Kosorukov V.1
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Afiliações:
- Blokhin Russian Cancer Research Center
- Edição: Volume 81, Nº 3 (2016)
- Páginas: 187-200
- Seção: Review
- URL: https://journals.rcsi.science/0006-2979/article/view/150789
- DOI: https://doi.org/10.1134/S0006297916030019
- ID: 150789
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Resumo
Virtually all recombinant proteins are now prepared using fusion domains also known as “tags”. The use of tags helps to solve some serious problems: to simplify procedures of protein isolation, to increase expression and solubility of the desired protein, to simplify protein refolding and increase its efficiency, and to prevent proteolysis. In this review, advantages and disadvantages of such fusion tags are analyzed and data on both well-known and new tags are generalized. The authors own data are also presented.
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Sobre autores
E. Kosobokova
Blokhin Russian Cancer Research Center
Autor responsável pela correspondência
Email: ekkos@mail.ru
Rússia, Moscow, 115478
K. Skrypnik
Blokhin Russian Cancer Research Center
Email: ekkos@mail.ru
Rússia, Moscow, 115478
V. Kosorukov
Blokhin Russian Cancer Research Center
Email: ekkos@mail.ru
Rússia, Moscow, 115478
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