Overview of fusion tags for recombinant proteins
- Авторлар: Kosobokova E.1, Skrypnik K.1, Kosorukov V.1
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Мекемелер:
- Blokhin Russian Cancer Research Center
- Шығарылым: Том 81, № 3 (2016)
- Беттер: 187-200
- Бөлім: Review
- URL: https://journals.rcsi.science/0006-2979/article/view/150789
- DOI: https://doi.org/10.1134/S0006297916030019
- ID: 150789
Дәйексөз келтіру
Аннотация
Virtually all recombinant proteins are now prepared using fusion domains also known as “tags”. The use of tags helps to solve some serious problems: to simplify procedures of protein isolation, to increase expression and solubility of the desired protein, to simplify protein refolding and increase its efficiency, and to prevent proteolysis. In this review, advantages and disadvantages of such fusion tags are analyzed and data on both well-known and new tags are generalized. The authors own data are also presented.
Негізгі сөздер
Авторлар туралы
E. Kosobokova
Blokhin Russian Cancer Research Center
Хат алмасуға жауапты Автор.
Email: ekkos@mail.ru
Ресей, Moscow, 115478
K. Skrypnik
Blokhin Russian Cancer Research Center
Email: ekkos@mail.ru
Ресей, Moscow, 115478
V. Kosorukov
Blokhin Russian Cancer Research Center
Email: ekkos@mail.ru
Ресей, Moscow, 115478
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