Neuroprotective properties of endocannabinoids N-arachidonoyl dopamine and N-docosahexaenoyl dopamine examined in neuronal precursors derived from human pluripotent stem cells
- Authors: Novosadova E.V.1, Arsenyeva E.L.1, Manuilova E.S.1, Khaspekov L.G.2, Bobrov M.Y.3,4, Bezuglov V.V.3, Illarioshkin S.N.2, Grivennikov I.A.1
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Affiliations:
- Institute of Molecular Genetics
- Research Center of Neurology
- Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
- Kulakov Research Center of Obstetrics, Gynecology, and Perinatology
- Issue: Vol 82, No 11 (2017)
- Pages: 1367-1372
- Section: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151518
- DOI: https://doi.org/10.1134/S0006297917110141
- ID: 151518
Cite item
Abstract
Neuroprotective properties of endocannabinoids N-arachidonoyl dopamine (NADA) and N-docosahexaenoyl dopamine (DHDA) were examined in neuronal precursor cells differentiated from human induced pluripotent stem cells and subjected to oxidative stress. Both compounds exerted neuroprotective activity, which was enhanced by elevating the concentration of the endocannabinoids within the 0.1–10 μM range. However, both agents at 10 μM concentration showed a marked toxic effect resulting in death of ~30% of the cells. Finally, antagonists of cannabinoid receptors as well as the receptor of the TRPV1 endovanilloid system did not hamper the neuroprotective effects of these endocannabinoids.
About the authors
E. V. Novosadova
Institute of Molecular Genetics
Author for correspondence.
Email: novek-img@mail.ru
Russian Federation, Moscow, 123182
E. L. Arsenyeva
Institute of Molecular Genetics
Email: grivigan@mail.ru
Russian Federation, Moscow, 123182
E. S. Manuilova
Institute of Molecular Genetics
Email: grivigan@mail.ru
Russian Federation, Moscow, 123182
L. G. Khaspekov
Research Center of Neurology
Email: grivigan@mail.ru
Russian Federation, Moscow, 125367
M. Yu. Bobrov
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry; Kulakov Research Center of Obstetrics, Gynecology, and Perinatology
Email: grivigan@mail.ru
Russian Federation, Moscow, 117997; Moscow, 117997
V. V. Bezuglov
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
Email: grivigan@mail.ru
Russian Federation, Moscow, 117997
S. N. Illarioshkin
Research Center of Neurology
Email: grivigan@mail.ru
Russian Federation, Moscow, 125367
I. A. Grivennikov
Institute of Molecular Genetics
Author for correspondence.
Email: grivigan@mail.ru
Russian Federation, Moscow, 123182
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