Neuroprotective properties of endocannabinoids N-arachidonoyl dopamine and N-docosahexaenoyl dopamine examined in neuronal precursors derived from human pluripotent stem cells
- Autores: Novosadova E.1, Arsenyeva E.1, Manuilova E.1, Khaspekov L.2, Bobrov M.3,4, Bezuglov V.3, Illarioshkin S.2, Grivennikov I.1
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Afiliações:
- Institute of Molecular Genetics
- Research Center of Neurology
- Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
- Kulakov Research Center of Obstetrics, Gynecology, and Perinatology
- Edição: Volume 82, Nº 11 (2017)
- Páginas: 1367-1372
- Seção: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151518
- DOI: https://doi.org/10.1134/S0006297917110141
- ID: 151518
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Resumo
Neuroprotective properties of endocannabinoids N-arachidonoyl dopamine (NADA) and N-docosahexaenoyl dopamine (DHDA) were examined in neuronal precursor cells differentiated from human induced pluripotent stem cells and subjected to oxidative stress. Both compounds exerted neuroprotective activity, which was enhanced by elevating the concentration of the endocannabinoids within the 0.1–10 μM range. However, both agents at 10 μM concentration showed a marked toxic effect resulting in death of ~30% of the cells. Finally, antagonists of cannabinoid receptors as well as the receptor of the TRPV1 endovanilloid system did not hamper the neuroprotective effects of these endocannabinoids.
Sobre autores
E. Novosadova
Institute of Molecular Genetics
Autor responsável pela correspondência
Email: novek-img@mail.ru
Rússia, Moscow, 123182
E. Arsenyeva
Institute of Molecular Genetics
Email: grivigan@mail.ru
Rússia, Moscow, 123182
E. Manuilova
Institute of Molecular Genetics
Email: grivigan@mail.ru
Rússia, Moscow, 123182
L. Khaspekov
Research Center of Neurology
Email: grivigan@mail.ru
Rússia, Moscow, 125367
M. Bobrov
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry; Kulakov Research Center of Obstetrics, Gynecology, and Perinatology
Email: grivigan@mail.ru
Rússia, Moscow, 117997; Moscow, 117997
V. Bezuglov
Shemyakin−Ovchinnikov Institute of Bioorganic Chemistry
Email: grivigan@mail.ru
Rússia, Moscow, 117997
S. Illarioshkin
Research Center of Neurology
Email: grivigan@mail.ru
Rússia, Moscow, 125367
I. Grivennikov
Institute of Molecular Genetics
Autor responsável pela correspondência
Email: grivigan@mail.ru
Rússia, Moscow, 123182
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