Express Method for the Identification of Industrially Valuable Yeast

Resumo

A method is proposed for the rapid identification of the taxa of five industrially valuable yeast groups: Pichia pastoris, Yarrowia lipolytica, Schizosaccharomyces pombe, Saccharomyces cerevisiae, and Kluyveromyces lactis. The specific primers selected based on nucleotide sequences of genes encoding rRNA were used in PCR. The method was effective in the identification of either pure yeast cultures or a mixture of deposited yeast strain cultures. Thus, this method was shown to be potentially viable for the identification of the studied yeasts in microbial preparations at the stages of the construction of producing strains and in industrial fermentations.