Express Method for the Identification of Industrially Valuable Yeast
- 作者: Borshchevskaya L.N.1, Gordeeva T.L.1, Kalinina A.N.1, Sineoky S.P.1
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隶属关系:
- State Research Institute for Genetics and Selection of Industrial Microorganisms, National Research Center, Kurchatov Institute (NRC Kurchatov Institute, GosNIIgenetika)
- 期: 卷 54, 编号 7 (2018)
- 页面: 717-722
- 栏目: Producers, Biology, Selection, and Gene Engineering
- URL: https://journals.rcsi.science/0003-6838/article/view/152706
- DOI: https://doi.org/10.1134/S0003683818070025
- ID: 152706
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详细
A method is proposed for the rapid identification of the taxa of five industrially valuable yeast groups: Pichia pastoris, Yarrowia lipolytica, Schizosaccharomyces pombe, Saccharomyces cerevisiae, and Kluyveromyces lactis. The specific primers selected based on nucleotide sequences of genes encoding rRNA were used in PCR. The method was effective in the identification of either pure yeast cultures or a mixture of deposited yeast strain cultures. Thus, this method was shown to be potentially viable for the identification of the studied yeasts in microbial preparations at the stages of the construction of producing strains and in industrial fermentations.
作者简介
L. Borshchevskaya
State Research Institute for Genetics and Selection of Industrial Microorganisms, National Research Center,Kurchatov Institute (NRC Kurchatov Institute, GosNIIgenetika)
编辑信件的主要联系方式.
Email: larisa.larbor3@yandex.ru
俄罗斯联邦, Moscow, 117545
T. Gordeeva
State Research Institute for Genetics and Selection of Industrial Microorganisms, National Research Center,Kurchatov Institute (NRC Kurchatov Institute, GosNIIgenetika)
Email: larisa.larbor3@yandex.ru
俄罗斯联邦, Moscow, 117545
A. Kalinina
State Research Institute for Genetics and Selection of Industrial Microorganisms, National Research Center,Kurchatov Institute (NRC Kurchatov Institute, GosNIIgenetika)
Email: larisa.larbor3@yandex.ru
俄罗斯联邦, Moscow, 117545
S. Sineoky
State Research Institute for Genetics and Selection of Industrial Microorganisms, National Research Center,Kurchatov Institute (NRC Kurchatov Institute, GosNIIgenetika)
Email: larisa.larbor3@yandex.ru
俄罗斯联邦, Moscow, 117545
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