Prostate state under varicosis of the pelvis (experimental study)

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Abstract

BACKGROUND: Chronic pelvic pain syndrome / chronic abacterial prostatitis is one of the most common diseases in urological practice. Despite its frequency the pathogenesis of the disease remains poorly understood. It is known that the morphological manifestation of chronic inflammation is sclerosis, in the development of which the venous congestion of the prostate is also important.

AIM: To evaluate the effect of chronic venous congestion of the pelvis on the prostate in a chronic experiment.

MATERIALS AND METHODS: An original model of persistent chronic varicose of the pelvis has been created. The experiment was carried out on 35 mature rabbits weighing 2.6–3.2 kg. Animals were divided into 3 groups. Control (n = 5) study of the normal anatomy of animals. In group 2 (n = 15), the creation of a model: ligation of the v. sacralis mediana, in combination with administration of progesterone solution. In group 3 (n = 15), false surgery and progesterone administration. Duplex scanning of the pelvic veins was performed at 1, 3 and 6 months, the diameter of the veins and the reflux after manual compression was assessed. After removing the animals from the experiment histological examination and morphometry of the prostate tissue were carried out. In prostate samples the concentration of hydroxyproline was investigated as a marker of connective tissue development.

RESULTS: Venous congestion of the pelvic organs in animals in the second group negatively affected the structure of the prostate gland. Histological examination of the samples showed infiltration of the prostate tissue, edema of the stroma, a sharp expansion of the veins at the beginning of the experiment, later noted the proliferation of connective tissue, compression of the acini and a decrease in the secretory activity of the prostate gland. The results of morphometry showed an increase in the area of the stromal component to a greater extent due to an increase in the volume of connective tissue. There was a tendency to an increase in the concentration of hydroxyproline in the prostate tissue in animals of the 2nd group with the achievement of maximum values by the 6th month of the experiment. In animals of the 3rd group no significant changes in the structure of the prostate gland were revealed.

CONCLUSIONS: The morphological manifestation of chronic venous hyperemia is infiltration of the prostate stroma and sclerogenesis.

About the authors

Anton Y. Tsukanov

Omsk State Medical University

Author for correspondence.
Email: autt@mail.ru
ORCID iD: 0000-0002-3497-5856
SPIN-code: 9310-1220
Scopus Author ID: 57194497218
http://omsk-osma.ru/vracham-i-provizoram/centr-povysheniya-kvalifikacii-i-professional-noy-perepodgotovki-specialistov/kafedry-i-kursy-dpo/hirurgicheskih-bolezney-i-urologii-dpo/sotrudniki-kafedry

Dr. Sci. (Med.), Professor, Head of the Department of Surgical Diseases and Urology of Additional Professional Education

Russian Federation, 12, Lenina Street,Omsk, 644099

Nickolay V. Rudchenko

Omsk State Medical University

Email: nrudrus@gmail.com
ORCID iD: 0000-0002-0121-3425

Postgraduate Student

Russian Federation, 12, Lenina Street,Omsk, 644099

Alexandr N. Kuzovkin

Clinical Medical and Sanitary Hospital №9

Email: autt@mail.ru

Head of the pathological department

Russian Federation, Omsk

Daniyar S. Ahmetov

Omsk State Medical University

Email: dsahmetov@gmail.com

Postgraduate Student

Russian Federation, 12, Lenina Street,Omsk, 644099

Stepan F. Alyabushev

City Clinical Emergency Hospital No. 2

Email: alyabushev1992@mail.ru

Urologist

Russian Federation, Omsk

References

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2. Fig. 1. Micropreparation of the prostate gland of an animal of the 1st group: a – microphotograph of a normal prostate gland, scalloped contour, thin interlobular septa, a large amount of secretion; b – the image is isolated of connective tissue, processed in the ImageJ program; c – calculation of the area of connective tissue in the interface of the ImageJ program

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3. Fig. 2. Micropreparation of the prostate gland of an animal of the 2nd group 1 month after the beginning of the experiment: a – smoothed glandular contour, decreased secretory activity (white arrow), dilated intraprostatic veins (red arrow), hematoxylin-eosin staining, ×100; b – severe interlobular fibrosis (black arrow), perifocal fibrosis and thickened venule wall (white arrow), Mallory staining, ×100

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4. Fig. 3. Micropreparation of the prostate gland of an animal of the 2nd group 3 month after the beginning of the experiment: a, b – desquamated epithelium in the lumen of the acini (yellow arrow), pronounced interlobular and perifocal fibrosis (red arrow), dilated venules (blue arrow), a sharp decrease in the height of the epithelium (black arrow). Mallory staining, ×100; c – microphotograph of the altered prostate gland, smoothed contour, thick interlobular septa, no secretion; d – the image is isolated of connective tissue, processed in the ImageJ program; e – calculation of the area of connective tissue in the interface of the ImageJ program

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5. Fig. 4. Micropreparation of the prostate gland of an animal of the 2nd group 6 month after the beginning of the experiment: a – thickened fibrous layers squeeze the glandular structures (white arrow), individual groups of acini with reduced epithelial height (yellow arrow). Mallory staining, ×40; b – fibrotic changes in the acini of the prostate (white arrow), a sharply smoothed contour of the epithelium with no secretion in the lumen of the acini (black arrow). Mallory staining, ×100; c – microphotograph of the altered prostate gland with pronounced intraacinar fibrosis; d – the image is isolated of connective tissue, processed in the ImageJ program; e – calculation of the area of connective tissue in the interface of the ImageJ program

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