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Post a Comment Effects of a single introduction of pamidronate on intact bone under the conditions of the experimental model of the treatment of tuberculosis ostitis
- Authors: Petukhova V.V.1, Mushkin A.Y.1,2, Kostik M.M.3, Vinogradova T.I.1
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Affiliations:
- St. Petersburg Research Institute of Phthisiopulmonology
- Academician Pavlov First St. Petersburg State Medical University
- St. Petersburg State Pediatric Medical University
- Issue: Vol 13, No 6 (2022)
- Pages: 55-62
- Section: Original studies
- URL: https://journals.rcsi.science/pediatr/article/view/133041
- DOI: https://doi.org/10.17816/PED13655-62
- ID: 133041
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Abstract
BACKGROUND: Information about the features of intact bone tissue of the contralateral segment in the treatment of tuberculous osteitis is absent in the literature.
AIM: Study of the features of intact bone tissue in the conditions of complex treatment of experimental tuberculous osteitis.
MATERIALS AND METHODS: On an experimental model of tuberculous osteitis of the condyle of the right femur (Mycobacterium tuberculosis H37Rv) in 21 rabbits, the morphometric characteristics of the contralateral femurs, representatively selected from 4 groups, were studied: group (control) — who did not receive either surgical or medical treatment; groups 2–4 — radical surgical removal of the focus, supplemented: No. 2 — antituberculosis therapy, group 3 — antituberculosis therapy and a single injection of bisphosphonates (pamidronate), group 4 — a single injection of bisphosphonates. In autopsy material studied: the area of bone tissue, the thickness of bone beams, the number of osteoblasts, osteocytes, osteoclasts and the proportion of their active forms.
RESULTS: The following features were revealed in intact samples of different groups: group 1 — the minimum area of bone tissue and the thickness of the beams, the maximum number of osteoblasts, including active ones, the average number of osteocytes; groups 2 and 3 — average values of the area and thickness of bone beams, the average number of osteoblasts, the pool of osteocytes is represented mainly by inactive cells; groups 3 and 4 the minimum value of active osteoblasts; group 4 — the maximum thickness of the bone beams, a greater total number of osteoblasts and osteocytes. No osteoclasts were found in any group.
CONCLUSIONS: Different ratios of bone tissue area, thickness of bone beams, and osteoforming cellular elements in animals receiving different treatment indicate both the systemic effect of a specific process and the drug effect of anti-tuberculosis drugs and bisphosphonates on uninfected bones.
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##article.viewOnOriginalSite##About the authors
Veronika V. Petukhova
St. Petersburg Research Institute of Phthisiopulmonology
Email: nika_add@mail.ru
ORCID iD: 0000-0002-2358-5529
Postgraduate Student
Russian Federation, Saint PetersburgAlexander Yu. Mushkin
St. Petersburg Research Institute of Phthisiopulmonology; Academician Pavlov First St. Petersburg State Medical University
Email: aymushkin@mail.ru
ORCID iD: 0000-0002-1342-3278
MD, PhD, Dr Med Sci, Professor, Chief Researcher; Professor of the Department of Traumatology and Orthopedics
Russian Federation, Saint Petersburg; Saint PetersburgMikhail M. Kostik
St. Petersburg State Pediatric Medical University
Email: kostmikhail@yandex.ru
ORCID iD: 0000-0002-1180-8086
MD, PhD, Dr Med Sci, Professor of the Department of Hospital Pediatrics
Russian Federation, Saint PetersburgTatiana I. Vinogradova
St. Petersburg Research Institute of Phthisiopulmonology
Author for correspondence.
Email: vinogradova@spbniif.ru
ORCID iD: 0000-0002-5234-349X
MD, PhD, Dr Med Sci, Professor, Chief Researcher, coordinator of the direction “Experimental tuberculosis and innovative technologies”
Russian Federation, Saint PetersburgReferences
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