代谢性谷氨酸受体拮抗剂对大鼠最大电击模型影响的研究

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目的：目的：研究代谢型谷氨酸（mGlu）受体拮抗剂对大鼠脑内最大电刺激致惊厥发作发展及脂质过氧化产物含量的影响。

材料与方法。实验对象为体重180—210 g的雄性Wistar大鼠87只。实验中采用了最大电击法。MGlu受体第1和第5亚型的选择性拮抗剂在最大电击前1小时给予。对照组大鼠注射等量生理盐水。脂质过氧化过程的强度通过次级产物与硫代巴比妥酸反应的水平采用分光光度法进行评估。

结果。研究发现，最大电击程序导致大鼠大脑皮质中明显的阵挛-强直性惊厥发作和脂质过氧化产物水平增加3倍以上。研究发现，第5亚型受体mGlu选择性拮抗剂几乎完全阻断大鼠癫痫发作的紧张期，并在很大程度上阻止了最大电刺激引起的脂质过氧化过程的强化。44%的实验动物在给予第1亚型选择性mGlu受体拮抗剂后出现强直性惊厥。同时，由于电击的作用，这种拮抗剂也部分降低了脂质过氧化产物的含量。

结论。因此，代谢性谷氨酸受体参与了大鼠最大电击诱发惊厥发作的发生机制。同时，通过阻断第5亚型的mGlu受体，观察到惊厥表现最明显的减弱，以及最大电休克程序导致的脂质过氧化产物水平的升高。获得的数据证实了使用第5亚型的代谢受体拮抗剂作为潜在的抗惊厥药物治疗广泛性癫痫的可能性

作者简介

Valentina Bashkatova

P.K. Anokhin Scientific Research Institute of Normal Physiology

编辑信件的主要联系方式.
Email: v.bashkatova@nphys.ru
ORCID iD: 0000-0001-6632-5973
SPIN 代码: 7383-8483

MD, Dr.Sci.(Biol.), Leading Researcher of the Reinforcements Physiology Laboratory

俄罗斯联邦, Moscow

Sergey Sudakov

P.K. Anokhin Scientific Research Institute of Normal Physiology

Email: s-sudakov@nphys.ru
ORCID iD: 0000-0002-9485-3439
SPIN 代码: 1127-4090
Researcher ID: D-1647-2013

MD, Dr.Sci.(Med.), Professor, Director, Head of the Reinforcements Physiology Laboratory

俄罗斯联邦, Moscow

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